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Legerstee, Karin (author), Sueters, J. (author), Abraham, Tsion E. (author), Slotman, Johan A. (author), Kremers, Gert Jan (author), Hoogenboom, J.P. (author), Houtsmuller, A.B. (author)Focal adhesions (FAs) are the main cellular structures to link the intracellular cytoskeleton to the extracellular matrix. FAs mediate cell adhesion, are important for cell migration and are involved in many (patho)-physiological processes. Here we examined FAs and their associated actin fibres using correlative fluorescence and scanning...journal article 2022
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Inamdar, A.S. (author), Gromala, Przemyslaw Jakub (author), Prisacaru, Alexandru (author), Kabakchiev, Alexander (author), Yang, Yu Hsiang (author), Han, Bongtae (author)Epoxy molding compound (EMC) is widely used for encapsulating automotive electronics. Among all of the components of an electronic package, EMC is most exposed to the atmosphere, and thus undergoes aging. During high-temperature operation, EMC is oxidized, which alters its mechanical properties, and thus can affect the reliability of electronic...book chapter 2022
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Hulleman, C.N. (author)<i>Localization microscopy has circumvented the diffraction limit by sequentially</i> imaging individual light emitting molecules at a time. The position of these individual molecules can be determined and a super-resolution reconstruction is made with improved resolution. Normally freely rotating emitters are used such that the point spread...doctoral thesis 2021
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Maduro, L.A. (author)The family of transitionmetal dichalcogenides offer a unique platformfor electronic and optical tunability due to the sensitivity to their dimensional configuration, edge terminations, and varying crystal phases. In this thesis we focus on structures based on the transition metal dichalcogenides MoS2 and WS2. We study how different crystal...doctoral thesis 2021
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Bijster, R.J.F. (author)In this thesis a proof-of-principle demonstration is developed that uses the heat flux between a probe and a sample as a proxy for their separation. The proposed architecture uses a probe that consists of a bilayer cantilever with an attached sphere at its free end. The deflection of the cantilever that is caused by the heat input is measured...doctoral thesis 2021
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Dreesens, L.L. (author)Phages are nanomachines composed of a protein coat encapsulating a genome. Since they are metabolically inert, they depend on a bacterial host for replication. They are abundantly present in all kinds of environments, patiently awaiting their target. The nanoscopic mechanical details of how phages or phage-like particles find the correct target...doctoral thesis 2021
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Krielaart, M.A.R. (author)We propose a microscopy scheme for the controlled modulation of the electron wave front that utilizes patterned electron mirrors. The ability to control the wave front of the electron finds many applications in electron microscopy, for instance in contrast enhancement techniques, beam mode conversion, low-dose imaging techniques such as quantum...doctoral thesis 2021
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Kamat, Ameya (author), Lubelli, B. (author), Schlangen, E. (author)Sodium chloride (NaCl) is one of the ubiquitous soluble salts in the environment and is responsible for weathering of building materials. The salt weathering is attributed to the stress developed from crystallisation of these salts in pores of the building materials, with supersaturation as the driving force. In the last years, researchers have...conference paper 2021
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Kundarapu, Laxman Kumar (author), Choudhury, Satyajeet (author), Acharya, Sriprasad (author), Vatti, Anoop Kishore (author), Pandiyan, Sudharsan (author), Gadag, Shivaprasad (author), Nayak, Usha Yogendra (author), Dey, P. (author)The aggregation behavior of asphaltene in the toluene-hexane mixture is systematically investigated using experimental techniques such as optical microscopy and Fourier-transform infrared spectroscopy (FTIR) combined with molecular dynamics (MD) simulations. The optical images of various asphaltene concentrations are processed to determine...journal article 2021
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Balasubramani, Vinoth (author), Kujawińska, Małgorzata (author), Allier, Cédric (author), Anand, Vijayakumar (author), Cheng, Chau Jern (author), Depeursinge, Christian (author), Hai, Nathaniel (author), Kalkman, J. (author), Park, Yong Keun (author)Quantitative Phase Imaging (QPI) provides unique means for the imaging of biological or technical microstructures, merging beneficial features identified with microscopy, interferometry, holography, and numerical computations. This roadmap article reviews several digital holography-based QPI approaches developed by prominent research groups....review 2021
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Navikas, Vytautas (author), Descloux, Adrien C. (author), Grußmayer, K.S. (author), Marion, Sanjin (author), Radenovic, Aleksandra (author)A variety of modern super-resolution microscopy methods provide researchers with previously inconceivable biological sample imaging opportunities at a molecular resolution. All of these techniques excel at imaging samples that are close to the coverslip, however imaging at large depths remains a challenge due to aberrations caused by the...journal article 2021
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Neumann, Elizabeth K. (author), Patterson, Nathan Heath (author), Allen, Jamie L. (author), Migas, L.G. (author), Yang, Haichun (author), Brewer, Maya (author), Anderson, David M. (author), Harvey, Jennifer (author), Harris, Raymond C. (author), Van de Plas, Raf (author)Here, we describe the preservation and preparation of human kidney tissue for interrogation by histopathology, imaging mass spectrometry, and multiplexed immunofluorescence. Custom image registration and integration techniques are used to create cellular and molecular atlases of this organ system. Through careful optimization, we ensure high...journal article 2021
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Smith, Gregory N. (author), Brok, Erik (author), Schmiele, Martin (author), Mortensen, Kell (author), Bouwman, W.G. (author), Duif, C.P. (author), Hassenkam, Tue (author), Alm, Martin (author), Thomsen, Peter (author), Arleth, Lise (author)By introducing hydrophilic polymers into silicone medical devices, highly beneficial biomedical properties can be realized. An established solution to introduce hydrophilic polymers is to form an interpenetrating polymer network (IPN) by performing the hydrogel synthesis in the presence of silicone swollen in supercritical carbon dioxide. The...journal article 2021
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Lane, R. (author), Vos, Y. (author), Wolters, A.E. (author), van Kessel, L.C.P.M. (author), Chen, S. Elisa (author), Liv, Nalan (author), Klumperman, Judith (author), Giepmans, Ben N.G. (author), Hoogenboom, J.P. (author)Large-scale electron microscopy (EM) allows analysis of both tissues and macromolecules in a semi-automated manner, but acquisition rate forms a bottleneck. We reasoned that a negative bias potential may be used to enhance signal collection, allowing shorter dwell times and thus increasing imaging speed. Negative bias potential has previously...journal article 2021
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Legerstee, W.J. (author), Boekel, M.G.M. (author), Boonstra, S. (author), Kelder, E.M. (author)An Atomic Force Microscope (AFM) is combined with a special designed glovebox system and coupled to a Galvanostat/Potentiostat to allow measurements on electrochemical properties for battery research. An open cell design with electrical contacts makes it possible to reach the electrode surface with the cantilever so as to perform measurements...journal article 2021
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Srinivasa Raja, A. (author), de Boer, Pascal (author), Giepmans, Ben N.G. (author), Hoogenboom, J.P. (author)Electron microscopy is crucial for imaging biological ultrastructure at nanometer resolution. However, electron irradiation also causes specimen damage, reflected in structural and chemical changes that can give rise to alternative signals. Here, luminescence induced by electron-beam irradiation is reported across a range of materials widely...journal article 2021
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Storteboom, J. (author), Barbiero, Martina (author), Castelletto, Stefania (author), Gu, Min (author)The negatively charged nitrogen-vacancy (NV <sup>-</sup>) centre in nanodiamonds (NDs) has been recently studied for applications in cellular imaging due to its better photo-stability and biocompatibility if compared to other fluorophores. Super-resolution imaging achieving 20-nm resolution of NV <sup>-</sup> in NDs has been proved over the...journal article 2021
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Van Der Burgt, Julia S. (author), Dieleman, Christian D. (author), Johlin, Eric (author), Geuchies, J.J. (author), Houtepen, A.J. (author), Ehrler, Bruno (author), Garnett, Erik C. (author)Accurately controlling light emission using nano- and microstructured lenses and antennas is an active field of research. Dielectrics are especially attractive lens materials due to their low optical losses over a broad bandwidth. In this work we measure highly directional light emission from patterned quantum dots (QDs) aligned underneath...journal article 2021
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Xia, Fei (author), Gevers, Monique (author), Fognini, Andreas (author), Mok, Aaron T. (author), Li, Bo (author), Akbari, Najva (author), Esmaeil Zadeh, I.Z. (author), Qin-Dregely, Y. (author), Xu, Chris (author)Optical microscopy is a valuable tool for in vivo monitoring of biological structures and functions because of its noninvasiveness. However, imaging deep into biological tissues is challenging due to the scattering and absorption of light. Previous research has shown that the two optimal wavelength windows for high-resolution deep mouse brain...journal article 2021
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Maduro, L.A. (author), Noordam, M.L. (author), Bolhuis, M. (author), Kuipers, L. (author), Conesa Boj, S. (author)The fabrication of 2D materials, such as transition metal dichalcogenides (TMDs), in geometries beyond the standard platelet-like configuration exhibits significant challenges which severely limit the range of available morphologies. These challenges arise due to the anisotropic character of their bonding van der Waals out-of-plane while...journal article 2021