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Onderzoek naar de mogelijke duurzaamheidsingrepen bij het duurzaam transformeren van industrieel erfgoed. Hierbij wordt gekeken naar de motieven van de opdrachtgever om in te spelen op duurzaamheid en de haalbaarheid van duurzame transformatie. Uiteindelijk worden scenario's met bijbehorende aandachtspunten geformuleerd. - Research on possible sustainability aspects that can be used in a sustainable building transformation of industrial heritage. Motives from the initiator to make use of sustainability aspects and the feasability of the sustianable building transformation are taken into account. Different scenarios and their issues are formulated.

Motivation: Synthetic biology aims at building biological systems for useful purposes. Relatively simple gene networks have been engineered, but the design process is limited. Many papers advocate the use of engineering concepts like standardization and modular design to simplify the design process and enable the design of more complex systems. Currently, there are no tools available that implement both concepts in a practical way. Results: We have developed a software tool to show how standardization and modular design can be used for the design of logic gene networks. We introduce gene network templates to be able to use modular design in a practical way and use a standard model to simplify the design process and enable reuse of parameters. We have designed three logic gate templates and used them to build two logic gene networks: a demultiplexer and a D-latch. Our software tool was used to turn the templates into devices and to evaluate the performance of the devices. The results show that the devices are evaluated correctly. Furthermore, the results show that for the design of a gene network our method can be used to indicate which biological parts are preferred at what location in the network.

The design formula for rubble mound breakwaters by Van der Meer has an unclear Notional Permeability term. This term causes a lot of confusion for designers. In the past many people have tried to derive a better formulation for that term by experimental and analytical research. The goal of this study was to obtain a better formulation along a numerical way. This study explores the numerical possibilities and tries to define which direction has to be taken in future research. As a first step, a very simplified case is taken with a vertical homogeneous breakwater which interact with monochromatic waves. In total six different blocks were made of epoxy and elastocoast. Only 4 out of the 6 blocks were tested. Also the porosity (n), laminar friction (α) and turbulent friction constant (β) of the blocks were determined experimentally. This way the experimental results could be compared with computations. These experiments have been done in the large flume of the Environmental Fluid Mechanics Laboratory of the TU Delft. Two types of data were collected: pore pressures and water levels in front and behind the block. The water levels seemed to be the most reliable data. The main deficit of the setup was the wave absorber at the end of the flume. The wave absorber is not able to sufficiently absorb long waves. So the dataset had to be corrected for that effect. The created dataset was in line with results from earlier experiments. Results were compared with an analytical solution and the numerical SWASH model. Comparisons with the analytical solution showed a reasonable fit without any calibration. The SWASH model showed in first instance large deviations using the same dataset. By calibrating the turbulent flow resistance β, it was possible to generate a decent fit. However, the used β constants are 6-10 times higher than the measured β constants. This is physically unrealistic high. Therefore the most likely explanation is an error in the transition between the water and the porous medium. During the experiment discontinuities can occur on this transition while SWASH uses an continuity requirement. Numerical tests were performed on some multi-layered combinations of the different blocks in order to derive a "Vertical P" value in a similar way as Van der Meer determined his P=0.4 structure. The results showed, nevertheless, quite some different patterns as the computations done by Van der Meer. However, taking into account all the problems with calibrating the SWASH model the results for the notional permeability seemed very promising. This numerical method shows the possibility of numerically calculating a notional permeability and should be investigated further in the future.

After its founding 15 years ago, Tam Tam has encountered the limits of legacy systems used for the internal processes in recent years. To overcome these limits, multiple efforts have been made to enforce a transition to specific Enterprise Application Integration styles. Although the efforts resulted in working application interactions, the desire for an organized whole sustained. The motivation for this thesis project was to make one final effort towards a new architecture by providing a carte blanche for all steps to be taken as well as their outcomes. The main goal of this thesis project was to research the most logical „next step‟ for Tam Tam to take with the internal systems architecture and to derive which improvements this step brings into the picture. To reach this goal, a crossroad of three key elements is identified. 1. Context, the first element is to figure out what the current architectural landscape looks like and what can be learned from the previous attempts at integrating the systems. 2. Theory, the second element is to figure out how architectures can be compared, what the possible solutions are and which of those fits Tam Tam best. 3. Practice, the third element is to link the theoretical design to the practice to assess its feasibility, to evaluate which improvements for Tam Tam are introduced and, finally, to evaluate the chosen methodology. The choice for sub-steps in the chosen methodology is based on best practices and guidelines from available (relevant) literature and is adapted to be used in this specific context. The focus of this adaption is that the chosen steps must be adaptable to other contexts too. After the complete methodology has been carried out, the key deliverables can be divided into two different categories: science and Tam Tam. For science the main deliverables are: 1. An aggregated list of re-usable insights 2. A case study for an Enterprise Service Bus transition 3. Are-usable step-by-step approach from legacy systems architecture towards the „most logical‟ next architecture. The key deliverables for Tam Tam entail: A. A conclusion of the continuous effort towards Enterprise Application Integration B. Sellable BizTalk know-how C. An improved internal architecture with regard to flexibility, maintainability and adaptability Concluding, the given carte blanche allowed for a successful path towards the best fitting Enterprise Application Integration style for Tam Tam. Furthermore, the methodology used is assembled in such a way it can withstand usage in other contexts and can be seen as a first step towards a formal definition of said methodology. This re- usability is introduced by the context-free focus during the definition of said methodology.

Document(en) uit de collectie Chemische Procestechnologie

Protein sequence features are explored in relation to the production of over-expressed extracellular proteins by fungi. Knowledge on features influencing protein production and secretion could be employed to improve enzyme production levels in industrial bioprocesses via protein engineering. A large set, over 600 homologous and nearly 2,000 heterologous fungal genes, were overexpressed in Aspergillus niger using a standardized expression cassette and scored for high versus no production. Subsequently, sequence-based machine learning techniques were applied for identifying relevant DNA and protein sequence features. The amino-acid composition of the protein sequence was found to be most predictive and interpretation revealed that, for both homologous and heterologous gene expression, the same features are important: tyrosine and asparagine composition was found to have a positive correlation with high-level production, whereas for unsuccessful production, contributions were found for methionine and lysine composition. The predictor is available online at http://bioinformatics.tudelft.nl/hipsec. Subsequent work aims at validating these findings by protein engineering as a method for increasing expression levels per gene copy.

In the current society, logistics is faced with at least two big challenges. The first challenge considers safety and security measurements dealing with terrorism, smuggling, and related security accidents with a high societal impact. The second challenge is to meet sustainability requirements implying optimal use of resources and physical infrastructure. A condition sine qua non for dealing with these challenges is the realization of a flexible platform for sustainable and secure data exchange between collaborating global supply chain actors. This paper presents such a platform. It allows shippers, authorities, logistic service providers, and carriers to be fully interoperable across closed communities, to perform paperless logistics, and to adhere to societal demands of security, safety, and sustainability. Its functionality is derived from similar approaches as have been applied in modern social media. The paper elaborates on the functionality of the platform and its implications for research. It builds upon the research and innovation work as currently done within the EU FP7 Cassandra and iCargo projects.

An intermediate frequency (IF) band digitizing radiometer system in the 100–200 GHz frequency range has been developed for Tokamak diagnostics and control, and other fields of research which require a high flexibility in frequency resolution combined with a large bandwidth and the retrieval of the full wave information of the mm-wave signals under investigation. The system is based on directly digitizing the IF band after down conversion. The enabling technology consists of a fast multi-giga sample analog to digital converter that has recently become available. Field programmable gate arrays (FPGA) are implemented to accomplish versatile real-time data analysis. A prototype system has been developed and tested and its performance has been compared with conventional electron cyclotron emission (ECE) spectrometer systems. On the TEXTOR Tokamak a proof of principle shows that ECE, together with high power injected and scattered radiation, becomes amenable to measurement by this device. In particular, its capability to measure the phase of coherent signals in the spectrum offers important advantages in diagnostics and control. One case developed in detail employs the FPGA in real-time fast Fourier transform (FFT) and additional signal processing. The major benefit of such a FFT-based system is the real-time trade-off that can be made between frequency and time resolution. For ECE diagnostics this corresponds to a flexible spatial resolution in the plasma, with potential application in smart sensing of plasma instabilities such as the neoclassical tearing mode (NTM) and sawtooth instabilities. The flexible resolution would allow for the measurement of the full mode content of plasma instabilities contained within the system bandwidth.