Print Email Facebook Twitter Quantitative Superresolution Microscopy Reveals Differences in Nuclear DNA Organization of Multiple Myeloma and Monoclonal Gammopathy of Undetermined Significance Title Quantitative Superresolution Microscopy Reveals Differences in Nuclear DNA Organization of Multiple Myeloma and Monoclonal Gammopathy of Undetermined Significance Author Sathitruangsak, C. Righolt, C.H. Klewes, L. Tammur, P. Ilus, T. Tamm, A. Punab, M. Olujohungbe, A. Mai, S. Faculty Applied Sciences Department ImPhys/Imaging Physics Date 2015-03-09 Abstract The mammalian nucleus has a distinct substructure that cannot be visualized directly by conventional microscopy. In this study, the organization of the DNA within the nucleus of multiple myeloma (MM) cells, their precursor cells (monoclonal gammopathy of undetermined significance; MGUS) and control lymphocytes of the representative patients is visualized and quantified by superresolution microscopy. Three-dimensional structured illumination microscopy (3D-SIM) increases the spatial resolution beyond the limits of conventional widefield fluorescence microscopy. 3D-SIM reveals new insights into the nuclear architecture of cancer as we show for the first time that it resolves organizational differences in intranuclear DNA organization of myeloma cells in MGUS and in MM patients. In addition, we report a significant increase in nuclear submicron DNA structure and structure of the DNA-free space in myeloma nuclei compared to normal lymphocyte nuclei. Our study provides previously unknown details of the nanoscopic DNA architecture of interphase nuclei of the normal lymphocytes, MGUS and MM cells. This study opens new avenues to understanding the disease progression from MGUS to M Subject 3D structured illumination microscopyDNA nano structuremultiple myelomamonoclonal gammopathy of undertermined significance To reference this document use: http://resolver.tudelft.nl/uuid:01395c2e-d0bb-44c7-9565-0502447e57a4 DOI https://doi.org/10.1002/jcb.25030 Publisher Wiley ISSN 0730-2312 Source Journal of Cellular Biochemistry, 116 (5), 2015 Part of collection Institutional Repository Document type journal article Rights © 2014 Wiley Periodicals, Inc. Files PDF Righolt_2015.pdf 353.36 KB Close viewer /islandora/object/uuid:01395c2e-d0bb-44c7-9565-0502447e57a4/datastream/OBJ/view