Template-free 2D particle fusion in localization microscopy

Template-free 2D particle fusion in localization microscopy

Heydarian, H. (TU Delft ImPhys/Quantitative Imaging)
Schueder, Florian (Ludwig Maximilians University; Max Planck Institute of Biochemistry)
Strauss, Maximilian T. (Ludwig Maximilians University; Max Planck Institute of Biochemistry)
van Werkhoven, Ben (Netherlands eScience Center)
Fazel, Mohamadreza (University of New Mexico)
Lidke, K.A. (University of New Mexico)
Jungmann, Ralf (Ludwig Maximilians University; Max Planck Institute of Biochemistry)
Stallinga, S. (TU Delft ImPhys/Imaging Physics)
Rieger, B. (TU Delft ImPhys/Quantitative Imaging)

ImPhys/Imaging Physics

2018

Methods that fuse multiple localization microscopy images of a single structure can improve signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity to registration errors. We present a template-free particle-fusion approach based on an all-to-all registration that provides robustness against individual misregistrations and underlabeling. We achieved 3.3-nm Fourier ring correlation (FRC) image resolution by fusing 383 DNA origami nanostructures with 80% labeling density, and 5.0-nm resolution for structures with 30% labeling density.

http://resolver.tudelft.nl/uuid:0b2964e1-39af-4df9-8cd4-42e4ad7ddfd5

https://doi.org/10.1038/s41592-018-0136-6

2019-03-17

1548-7091

Nature Methods (online), 15, 781-784

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

Institutional Repository

journal article

© 2018 H. Heydarian, Florian Schueder, Maximilian T. Strauss, Ben van Werkhoven, Mohamadreza Fazel, K.A. Lidke, Ralf Jungmann, S. Stallinga, B. Rieger