Print Email Facebook Twitter Spatiotemporal control of coacervate formation within liposomes Title Spatiotemporal control of coacervate formation within liposomes Author Deshpande, S.R. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Brandenburg, Frank (Kavli institute of nanoscience Delft; Student TU Delft) Lau, A.W.H.R. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Last, M.G.F. (Kavli institute of nanoscience Delft; Student TU Delft) Spoelstra, W.K. (Kavli institute of nanoscience Delft; Student TU Delft) Reese, L. (TU Delft BN/Marileen Dogterom Lab; Kavli institute of nanoscience Delft) Wunnava, Sreekar Dogterom, A.M. (TU Delft BN/Bionanoscience; Kavli institute of nanoscience Delft) Dekker, C. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Department BN/Bionanoscience Date 2019 Abstract Liquid-liquid phase separation (LLPS), especially coacervation, plays a crucial role in cell biology, as it forms numerous membraneless organelles in cells. Coacervates play an indispensable role in regulating intracellular biochemistry, and their dysfunction is associated with several diseases. Understanding of the LLPS dynamics would greatly benefit from controlled in vitro assays that mimic cells. Here, we use a microfluidics-based methodology to form coacervates inside cell-sized (~10 µm) liposomes, allowing control over the dynamics. Protein-pore-mediated permeation of small molecules into liposomes triggers LLPS passively or via active mechanisms like enzymatic polymerization of nucleic acids. We demonstrate sequestration of proteins (FtsZ) and supramolecular assemblies (lipid vesicles), as well as the possibility to host metabolic reactions (β-galactosidase activity) inside coacervates. This coacervate-in-liposome platform provides a versatile tool to understand intracellular phase behavior, and these hybrid systems will allow engineering complex pathways to reconstitute cellular functions and facilitate bottom-up creation of synthetic cells. To reference this document use: http://resolver.tudelft.nl/uuid:13af606f-b140-4120-ab80-3eb071bbb9fa DOI https://doi.org/10.1038/s41467-019-09855-x ISSN 2041-1723 Source Nature Communications, 10 (1) Part of collection Institutional Repository Document type journal article Rights © 2019 S.R. Deshpande, Frank Brandenburg, A.W.H.R. Lau, M.G.F. Last, W.K. Spoelstra, L. Reese, Sreekar Wunnava, A.M. Dogterom, C. Dekker Files PDF s41467_019_09855_x.pdf 3.46 MB Close viewer /islandora/object/uuid:13af606f-b140-4120-ab80-3eb071bbb9fa/datastream/OBJ/view