Print Email Facebook Twitter Spectroscopic investigation of catalytic intermediates of the Chlorite Dismutase from Azospira oryzae Title Spectroscopic investigation of catalytic intermediates of the Chlorite Dismutase from Azospira oryzae: a treasure hunt Author Püschmann, Julia (TU Delft Applied Sciences) Contributor Hagedoorn, P.L. (mentor) Hagen, Wilfred R. (graduation committee) Pabst, M. (graduation committee) Degree granting institution Delft University of Technology Programme Life Science and Technology (LST) Date 2019-06-21 Abstract Anthropogenic activities like chemical industries are releasing chlorinated species, such as perchlorate (ClO4-) or chlorite (ClO2-), into the environment. These substances are toxic and difficult to treat in the wastewater. However, nature offers a way to solve this problem. Perchlorate-respiring bacteria possess an enzyme, namely called Chlorite dismutase (Cld). It can detoxify chlorite (ClO2-) - a by-product of the perchlorate respiration - to chloride (Cl-) and molecular oxygen (O2). Therefore, it is interesting to characterise Clds in more detail to resolve its reaction mechanism and to investigate its potential for wastewater treatment.This work presents new and direct evidence for transient high valent heme species during the reaction mechanism of an interesting enzyme, which can be applied for the bioremediation of chlorite. Subject Pre-steady-state kineticsChlorite DismutaseUltrafast spectroscopy To reference this document use: http://resolver.tudelft.nl/uuid:26fbf6f7-faa4-4ff8-aa70-2b4dde28ea4a Embargo date 2022-07-31 Part of collection Student theses Document type master thesis Rights © 2019 Julia Püschmann Files PDF MEP_Julia_P_schmann.pdf 4.19 MB Close viewer /islandora/object/uuid:26fbf6f7-faa4-4ff8-aa70-2b4dde28ea4a/datastream/OBJ/view