Print Email Facebook Twitter Biocompatible Graphene Oxide Nanosheets Densely Functionalized with Biologically Active Molecules for Biosensing Applications Title Biocompatible Graphene Oxide Nanosheets Densely Functionalized with Biologically Active Molecules for Biosensing Applications Author Lehner, B. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft) Benz, D. (TU Delft ChemE/Product and Process Engineering; TU Delft ChemE/Chemical Engineering) Moshkalev, Stanislav A. (University of Campinas) Meyer, Anne S. (University of Rochester) Cotta, Monica A. (University of Campinas) Janissen, R. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Department ChemE/Chemical Engineering Date 2021 Abstract Graphene oxide (GO) has immense potential for widespread use in diverse in vitro and in vivo biomedical applications owing to its thermal and chemical resistance, excellent electrical properties and solubility, and high surface-to-volume ratio. However, development of GO-based biological nanocomposites and biosensors has been hampered by its poor intrinsic biocompatibility and difficult covalent biofunctionalization across its lattice. Many studies exploit the strategy of chemically modifying GO by noncovalent and reversible attachment of (bio)molecules or sole covalent biofunctionalization of residual moieties at the lattice edges, resulting in a low coating coverage and a largely bioincompatible composite. Here, we address these problems and present a facile yet powerful method for the covalent biofunctionalization of GO using colamine (CA) and the poly(ethylene glycol) cross-linker that results in a vast improvement in the biomolecular coating density and heterogeneity across the entire GO lattice. We further demonstrate that our biofunctionalized GO with CA as the cross-linker provides superior nonspecific biomolecule adhesion suppression with increased biomarker detection sensitivity in a DNA-biosensing assay compared to the (3-aminopropyl)triethoxysilane cross-linker. Our optimized biofunctionalization method will aid the development of GO-based in situ applications including biosensors, tissue nanocomposites, and drug carriers. Subject APTESbioconjugationcolamineDNA biosensorgraphene oxidepoly(ethylene glycol)surface chemistry To reference this document use: http://resolver.tudelft.nl/uuid:2894b642-3ac9-4727-93f1-8ba855d2d10b DOI https://doi.org/10.1021/acsanm.1c01522 ISSN 2574-0970 Source ACS Applied Nano Materials, 4 (8), 8334-8342 Part of collection Institutional Repository Document type journal article Rights © 2021 B. Lehner, D. Benz, Stanislav A. Moshkalev, Anne S. Meyer, Monica A. Cotta, R. Janissen Files PDF acsanm.1c01522.pdf 3.19 MB Close viewer /islandora/object/uuid:2894b642-3ac9-4727-93f1-8ba855d2d10b/datastream/OBJ/view