Print Email Facebook Twitter Cellular responses of Saccharomyces cerevisiae at near-zero growth rates: Transcriptome analysis of anaerobic retentostat cultures Title Cellular responses of Saccharomyces cerevisiae at near-zero growth rates: Transcriptome analysis of anaerobic retentostat cultures Author Boender, L.G.M. Van Maris, A.J.A. De Hulster, E.A.F. Almering, M.J.H. Van der Klei, I.J. Veenhuis, M. De Winde, J.H. Pronk, J.T. Daran-Lapujade, P.A.S. Faculty Applied Sciences Department BT/Biotechnology Date 2011-09-26 Abstract Extremely low specific growth rates (below 0.01 h?1) represent a largely unexplored area of microbial physiology. In this study, anaerobic, glucose-limited retentostats were used to analyse physiological and genome-wide transcriptional responses of Saccharomyces cerevisiae to cultivation at near-zero specific growth rates. While quiescence is typically investigated as a result of carbon starvation, cells in retentostat are fed by small, but continuous carbon and energy supply. Yeast cells cultivated near-zero specific growth rates, while metabolically active, exhibited characteristics previously associated with quiescence, including accumulation of storage polymers and an increased expression of genes involved in exit from the cell cycle into G0. Unexpectedly, analysis of transcriptome data from retentostat and chemostat cultures showed, as specific growth rate was decreased, that quiescence-related transcriptional responses were already set in at specific growth rates above 0.025 h?1. These observations stress the need for systematic dissection of physiological responses to slow growth, quiescence, ageing and starvation and indicate that controlled cultivation systems such as retentostats can contribute to this goal. Furthermore, cells in retentostat do not (or hardly) divide while remaining metabolically active, which emulates the physiological status of metazoan post-mitotic cells. We propose retentostat as a powerful cultivation tool to investigate chronological ageing-related processes. Subject retentostatSaccharomyces cerevisiaenear-zero growth ratesquiescencechronological ageingOA-Fund TU Delft To reference this document use: http://resolver.tudelft.nl/uuid:32dc15c7-b033-4e1a-96ad-54146e190c21 DOI https://doi.org/10.1111/j.1567-1364.2011.00750.x Publisher Wiley ISSN 1567-1356 Source http://onlinelibrary.wiley.com/doi/10.1111/j.1567-1364.2011.00750.x/abstract Source FEMS Yeast Research, 11 (8), 2011 Part of collection Institutional Repository Document type journal article Rights (c) 2011 Federation of European Microbiological Societies Files PDF Daran-Lapujade.pdf 631 KB Close viewer /islandora/object/uuid:32dc15c7-b033-4e1a-96ad-54146e190c21/datastream/OBJ/view