The profound influence of lipid composition on the catalysis of the drug target nadh type ii oxidoreductase

Godoy Hernandez, A.; McMillan, D.G.G.

doi:10.3390/membranes11050363

The profound influence of lipid composition on the catalysis of the drug target nadh type ii oxidoreductase

Title

The profound influence of lipid composition on the catalysis of the drug target nadh type ii oxidoreductase

Author

Godoy Hernandez, A. (TU Delft BT/Biocatalysis)
McMillan, D.G.G. (TU Delft BT/Biocatalysis)

Date

2021

Abstract

Lipids play a pivotal role in cellular respiration, providing the natural environment in which an oxidoreductase interacts with the quinone pool. To date, it is generally accepted that negatively charged lipids play a major role in the activity of quinone oxidoreductases. By changing lipid compositions when assaying a type II NADH:quinone oxidoreductase, we demonstrate that phosphatidylethanolamine has an essential role in substrate binding and catalysis. We also reveal the importance of acyl chain composition, specifically c14:0, on membrane-bound quinone-mediated catalysis. This demonstrates that oxidoreductase lipid specificity is more diverse than originally thought and that the lipid environment plays an important role in the physiological catalysis of membrane-bound oxidoreductases.

Subject

Acyl chain
Biomimetic membrane
Lipids
Type II NADH:quinone oxidoreductase

To reference this document use:

http://resolver.tudelft.nl/uuid:382bf008-70af-424f-8d3a-14986cb359b9

DOI

https://doi.org/10.3390/membranes11050363

ISSN

2077-0375

Source

Membranes, 11 (5)

Part of collection

Institutional Repository