Print Email Facebook Twitter Image filtering in structured illumination microscopy using the Lukosz bound Title Image filtering in structured illumination microscopy using the Lukosz bound Author Righolt, C.H. Slotman, J.A. Young, I.T. Mai, S. Van Vliet, L.J. Stallinga, S. Faculty Applied Sciences Department IST/Imaging Science and Technology Date 2013-10-07 Abstract Various aspects of image filtering affect the final image quality in Structured Illumination Microscopy, in particular the regularization parameter and type of regularization function, the relative height of the side bands, and the shape of the apodization function. We propose an apodization filter without adjustable parameters based on the application of the Lukosz bound in order to guarantee a non-negative point spread function. Simulations of digital resolution charts and experimental data of chromatin structures and of actin filaments show artefact free reconstructions for a wide range of filter parameters. In general, a trade-off is observed between sharpness and noise suppression. Subject OA-Fund TU Delft To reference this document use: http://resolver.tudelft.nl/uuid:41eb8b94-c103-4d37-b8f3-3f41a53aa050 DOI https://doi.org/10.1364/OE.21.024431 Publisher Optical Society of America ISSN 1094-4087 Source http://www.opticsinfobase.org/oe/abstract.cfm?URI=oe-21-21-24431 Source Optics Express, 21 (21), 2013 Part of collection Institutional Repository Document type journal article Rights © 2013 Optical Society of America Files PDF Righolt_2013.pdf 3.1 MB Close viewer /islandora/object/uuid:41eb8b94-c103-4d37-b8f3-3f41a53aa050/datastream/OBJ/view