Print Email Facebook Twitter Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures Title Selection and subsequent physiological characterization of industrial Saccharomyces cerevisiae strains during continuous growth at sub- and- supra optimal temperatures Author Lip, K.Y.F. (TU Delft OLD BT/Cell Systems Engineering) García-Ríos, Estéfani (Institute of Agrochemistry and Food Technology) Costa, Carlos E. (University of Minho) Guillamón, José Manuel (Institute of Agrochemistry and Food Technology) Domingues, Lucília (University of Minho) Teixeira, José (University of Minho) van Gulik, W.M. (TU Delft OLD BT/Cell Systems Engineering) Date 2020 Abstract A phenotypic screening of 12 industrial yeast strains and the well-studied laboratory strain CEN.PK113-7D at cultivation temperatures between 12 °C and 40 °C revealed significant differences in maximum growth rates and temperature tolerance. From those 12, two strains, one performing best at 12 °C and the other at 40 °C, plus the laboratory strain, were selected for further physiological characterization in well-controlled bioreactors. The strains were grown in anaerobic chemostats, at a fixed specific growth rate of 0.03 h−1 and sequential batch cultures at 12 °C, 30 °C, and 39 °C. We observed significant differences in biomass and ethanol yields on glucose, biomass protein and storage carbohydrate contents, and biomass yields on ATP between strains and cultivation temperatures. Increased temperature tolerance coincided with higher energetic efficiency of cell growth, indicating that temperature intolerance is a result of energy wasting processes, such as increased turnover of cellular components (e.g. proteins) due to temperature induced damage. Subject ChemostatEnergetic efficiencySaccharomycesSBRTemperature tolerance To reference this document use: http://resolver.tudelft.nl/uuid:44fdad78-5545-4bb8-9841-edf8ed2c27e2 DOI https://doi.org/10.1016/j.btre.2020.e00462 Source Biotechnology Reports, 26 Part of collection Institutional Repository Document type journal article Rights © 2020 K.Y.F. Lip, Estéfani García-Ríos, Carlos E. Costa, José Manuel Guillamón, Lucília Domingues, José Teixeira, W.M. van Gulik Files PDF 1_s2.0_S2215017X20300175_main.pdf 1.01 MB Close viewer /islandora/object/uuid:44fdad78-5545-4bb8-9841-edf8ed2c27e2/datastream/OBJ/view