Print Email Facebook Twitter Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity Title Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity Author Wang, Xu Ping (South China University of Technology) Zhou, Peng Fei (South China University of Technology) Li, Zhi Gang (South China University of Technology) Yang, Bo (South China University of Technology) Hollmann, F. (TU Delft BT/Biocatalysis) Wang, Yong Hua (South China University of Technology) Date 2017-03-20 Abstract A Ser105Ala mutant of the lipase B from Candida antarctica enables 'perhydrolase-only' reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially eliminating the undesired hydrolysis reaction of the product. To reference this document use: http://resolver.tudelft.nl/uuid:48239759-6d0c-4aea-a203-a1313270040c DOI https://doi.org/10.1038/srep44599 ISSN 2045-2322 Source Scientific Reports, 7 Part of collection Institutional Repository Document type journal article Rights © 2017 Xu Ping Wang, Peng Fei Zhou, Zhi Gang Li, Bo Yang, F. Hollmann, Yong Hua Wang Files PDF srep44599.pdf 520.85 KB Close viewer /islandora/object/uuid:48239759-6d0c-4aea-a203-a1313270040c/datastream/OBJ/view