Global correction of optical distortions in multicolor single-molecule microscopy using Zernike polynomial gradients

Global correction of optical distortions in multicolor single-molecule microscopy using Zernike polynomial gradients

Mc Cluskey, K.A. (TU Delft BN/Nynke Dekker Lab; Kavli institute of nanoscience Delft)
van Veen, E.N.W. (TU Delft BN/Nynke Dekker Lab; Kavli institute of nanoscience Delft)
Cnossen, J.P. (TU Delft Team Carlas Smith)
Wesselink, W.J. (TU Delft BN/Nynke Dekker Lab; Kavli institute of nanoscience Delft)
Asscher, F.M. (TU Delft BN/Nynke Dekker Lab)
Smith, C.S. (TU Delft Team Carlas Smith; TU Delft ImPhys/Computational Imaging; TU Delft Delft Center for Systems and Control)
Dekker, N.H. (TU Delft BN/Nynke Dekker Lab; Kavli institute of nanoscience Delft)

Delft Center for Systems and Control

2021-12-06

Accurate image alignment is critical in multicolor single-molecule fluorescence microscopy. Global alignment using affine transformations leaves residual errors due to the nonlinearity of the distortions, which decreases the effective field of view. Subsequent local refinement demands either large amounts of reference data and processing time or specialized imaging techniques like active stabilization. Here, we present a global alignment method, S/T polynomial decomposition, that uses sums of Zernike polynomial gradients to decompose the distortion between two images, correcting both linear and nonlinear distortions simultaneously. With minimal reference data, we gain diagnostic information about the distortion and achieve a colocalization accuracy comparable to local registration methods across the entire field of view.

http://resolver.tudelft.nl/uuid:56c488a8-873c-4f43-886d-197deed7f94e

https://doi.org/10.1364/OE.445230

1094-4087

Optics Express, 29 (25), 42251-42264

Institutional Repository

journal article

© 2021 K.A. Mc Cluskey, E.N.W. van Veen, J.P. Cnossen, W.J. Wesselink, F.M. Asscher, C.S. Smith, N.H. Dekker