Localization microscopy at doubled precision with patterned illumination

Localization microscopy at doubled precision with patterned illumination

Cnossen, J.P. (TU Delft Team Raf Van de Plas; TU Delft ImPhys/Quantitative Imaging)
Hinsdale, T.A. (TU Delft ImPhys/Quantitative Imaging)
Thorsen, R.Ø. (TU Delft ImPhys/Quantitative Imaging)
Siemons, Marijn (Universiteit Utrecht)
Schueder, Florian (Ludwig Maximilians University; Max Planck Institute of Biochemistry)
Jungmann, Ralf (Ludwig Maximilians University; Max Planck Institute of Biochemistry)
Smith, C.S. (TU Delft ImPhys/Quantitative Imaging; TU Delft Team Raf Van de Plas; University of Oxford)
Rieger, B. (TU Delft ImPhys/Quantitative Imaging)
Stallinga, S. (TU Delft ImPhys/Imaging Physics)

ImPhys/Imaging Physics

2020

MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.

http://resolver.tudelft.nl/uuid:6449aea7-65ed-49e8-84fd-f7154629876d

https://doi.org/10.1038/s41592-019-0657-7

2019-06-09

1548-7091

Nature Methods (online), 17 (1), 59-63

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

Institutional Repository

journal article

© 2020 J.P. Cnossen, T.A. Hinsdale, R.Ø. Thorsen, Marijn Siemons, Florian Schueder, Ralf Jungmann, C.S. Smith, B. Rieger, S. Stallinga