Print Email Facebook Twitter Mariprofundus ferrooxydans PV-1 the First Genome of a Marine Fe(II) Oxidizing Zetaproteobacterium Title Mariprofundus ferrooxydans PV-1 the First Genome of a Marine Fe(II) Oxidizing Zetaproteobacterium Author Singer, E. Emerson, D. Webb, E.A. Barco, R.A. Kuenen, J.G. Nelson, W.C. Chan, C.S. Comolli, L.R. Ferriera, S. Faculty Applied Sciences Department Biotechnology Date 2011-09-23 Abstract Mariprofundus ferrooxydans PV-1 has provided the first genome of the recently discovered Zetaproteobacteria subdivision. Genome analysis reveals a complete TCA cycle, the ability to fix CO2, carbon-storage proteins and a sugar phosphotransferase system (PTS). The latter could facilitate the transport of carbohydrates across the cell membrane and possibly aid in stalk formation, a matrix composed of exopolymers and/or exopolysaccharides, which is used to store oxidized iron minerals outside the cell. Two-component signal transduction system genes, including histidine kinases, GGDEF domain genes, and response regulators containing CheY-like receivers, are abundant and widely distributed across the genome. Most of these are located in close proximity to genes required for cell division, phosphate uptake and transport, exopolymer and heavy metal secretion, flagellar biosynthesis and pilus assembly suggesting that these functions are highly regulated. Similar to many other motile, microaerophilic bacteria, genes encoding aerotaxis as well as antioxidant functionality (e.g., superoxide dismutases and peroxidases) are predicted to sense and respond to oxygen gradients, as would be required to maintain cellular redox balance in the specialized habitat where M. ferrooxydans resides. Comparative genomics with other Fe(II) oxidizing bacteria residing in freshwater and marine environments revealed similar content, synteny, and amino acid similarity of coding sequences potentially involved in Fe(II) oxidation, signal transduction and response regulation, oxygen sensation and detoxification, and heavy metal resistance. This study has provided novel insights into the molecular nature of Zetaproteobacteria. To reference this document use: http://resolver.tudelft.nl/uuid:744a0377-b8f0-461a-a591-803ac94f85c8 DOI https://doi.org/10.1371/journal.pone.0025386 Publisher Public Library of Science ISSN 1932-6203 Source http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0025386 Source Plos One, 6 (9), 2011 Part of collection Institutional Repository Document type journal article Rights © 2011 Singer et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Files PDF journal.pone.0025386.pdf 986.19 KB Close viewer /islandora/object/uuid:744a0377-b8f0-461a-a591-803ac94f85c8/datastream/OBJ/view