Print Email Facebook Twitter Similar temperature dependencies of glycolytic enzymes: An evolutionary adaptation to temperature dynamics? Title Similar temperature dependencies of glycolytic enzymes: An evolutionary adaptation to temperature dynamics? Author Cruz, L.A.B. Hebly, M. Duong, G.H. Wahl, S.A. Pronk, J.T. Heijnen, J.J. Daran-Lapujade, P. Van Gulik, W.M. Faculty Applied Sciences Department BT/Biotechnology Date 2012-12-07 Abstract Background Temperature strongly affects microbial growth, and many microorganisms have to deal with temperature fluctuations in their natural environment. To understand regulation strategies that underlie microbial temperature responses and adaptation, we studied glycolytic pathway kinetics in Saccharomyces cerevisiae during temperature changes. Results Saccharomyces cerevisiae was grown under different temperature regimes and glucose availability conditions. These included glucose-excess batch cultures at different temperatures and glucose-limited chemostat cultures, subjected to fast linear temperature shifts and circadian sinoidal temperature cycles. An observed temperature-independent relation between intracellular levels of glycolytic metabolites and residual glucose concentration for all experimental conditions revealed that it is the substrate availability rather than temperature that determines intracellular metabolite profiles. This observation corresponded with predictions generated in silico with a kinetic model of yeast glycolysis, when the catalytic capacities of all glycolytic enzymes were set to share the same normalized temperature dependency. Conclusions From an evolutionary perspective, such similar temperature dependencies allow cells to adapt more rapidly to temperature changes, because they result in minimal perturbations of intracellular metabolite levels, thus circumventing the need for extensive modification of enzyme levels. Subject glycolysiskinetic modellingmetabolomicssaccharomyces cerevisiaetemperature dynamics To reference this document use: http://resolver.tudelft.nl/uuid:75361b4a-7d5e-4ba2-a9ea-a1abb639c478 DOI https://doi.org/10.1186/1752-0509-6-151 Publisher BioMed Central ISSN 1752-0509 Source http://www.biomedcentral.com/1752-0509/6/151 Source BMC Systems Biology, 6, 2012 Part of collection Institutional Repository Document type journal article Rights © 2012 The Author(s)Licensee BioMed Central Ltd. - This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited Files PDF Wahl_2012.pdf 2.54 MB Close viewer /islandora/object/uuid:75361b4a-7d5e-4ba2-a9ea-a1abb639c478/datastream/OBJ/view