Print Email Facebook Twitter Formate Oxidase (FOx) from Aspergillus oryzae Title Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H 2 O 2 -Dependent Biocatalytic Oxidation Reactions Author Tieves, F. (TU Delft BT/Biocatalysis) Willot, S.J. (TU Delft BT/Biocatalysis) van Schie, M.M.C.H. (TU Delft BT/Biocatalysis) Rauch, M.C.R. (TU Delft BT/Biocatalysis) Younes, S.H.H. (TU Delft BT/Biocatalysis; Sohag University) Zhang, W. (TU Delft BT/Biocatalysis) Dong, J. (TU Delft BT/Biocatalysis) Gomez de Santos, P. (TU Delft BT/Biocatalysis) Hollmann, F. (TU Delft BT/Biocatalysis) Date 2019 Abstract An increasing number of biocatalytic oxidation reactions rely on H 2 O 2 as a clean oxidant. The poor robustness of most enzymes towards H 2 O 2 , however, necessitates more efficient systems for in situ H 2 O 2 generation. In analogy to the well-known formate dehydrogenase to promote NADH-dependent reactions, we here propose employing formate oxidase (FOx) to promote H 2 O 2 -dependent enzymatic oxidation reactions. Even under non-optimised conditions, high turnover numbers for coupled FOx/peroxygenase catalysis were achieved. Subject biocatalysisformate oxidasehydrogen peroxideoxidationoxyfunctionalisation To reference this document use: http://resolver.tudelft.nl/uuid:7cab5551-97fc-4dfd-89bd-9521b7af7c5c DOI https://doi.org/10.1002/anie.201902380 ISSN 1433-7851 Source Angewandte Chemie (International Edition), 58 (23), 7873-7877 Part of collection Institutional Repository Document type journal article Rights © 2019 F. Tieves, S.J. Willot, M.M.C.H. van Schie, M.C.R. Rauch, S.H.H. Younes, W. Zhang, J. Dong, P. Gomez de Santos, F. Hollmann Files PDF Tieves_et_al_2019_Angewan ... dition.pdf 1.14 MB Close viewer /islandora/object/uuid:7cab5551-97fc-4dfd-89bd-9521b7af7c5c/datastream/OBJ/view