Print Email Facebook Twitter The fluxes through glycolytic enzymes in Saccharomyces cerevisiae are predominantly regulated at posttranscriptional levels Title The fluxes through glycolytic enzymes in Saccharomyces cerevisiae are predominantly regulated at posttranscriptional levels Author Daran, J.M. Bakker, B.M. Pronk, J.T. Westerhoff, H.V. De Winde, J.H. Heck, A.J.R. Slijper, M. De Groot, M.J.L. Luttik, M.A.H. Van Gulik, W.M. Rossell, S. Daran-Lapujade, P. Faculty Applied Sciences Department Biotechnology Date 2007-10-02 Abstract Metabolic fluxes may be regulated ‘‘hierarchically,’’ e.g., by changes of gene expression that adjust enzyme capacities (Vmax) and/or ‘‘metabolically’’ by interactions of enzymes with substrates, products, or allosteric effectors. In the present study, a method is developed to dissect the hierarchical regulation into contributions by transcription, translation, protein degradation, and posttranslational modification. The method was applied to the regulation of fluxes through individual glycolytic enzymes when the yeast Saccharomyces cerevisiae was confronted with the absence of oxygen and the presence of benzoic acid depleting its ATP. Metabolic regulation largely contributed to the10-fold change in flux through the glycolytic enzymes. This contribution varied from 50 to 80%, depending on the glycolytic step and the cultivation condition tested. Within the 50–20% hierarchical regulation of fluxes, transcription played a minor role, whereas regulation of protein synthesis or degradation was the most important. These also contributed to 75–100% of the regulation of protein levels. Subject Open Access To reference this document use: http://resolver.tudelft.nl/uuid:87ac7063-88c8-4c8e-be69-a54505eb3678 DOI https://doi.org/10.1073/pnas.0707476104 Publisher National Academy of Sciences ISSN 1091-6490 Source PNAS Proceedings of the National Academy of Sciences, 104 (40), 2007 Part of collection Institutional Repository Document type journal article Rights (c) 2007 The Author(s) Files PDF DaranPNAS.pdf 304.57 KB Close viewer /islandora/object/uuid:87ac7063-88c8-4c8e-be69-a54505eb3678/datastream/OBJ/view