Print Email Facebook Twitter Evaluation of feasibility of using the bacteriophage T4 lysozyme to improve the hydrolysis and biochemical methane potential of secondary sludge Title Evaluation of feasibility of using the bacteriophage T4 lysozyme to improve the hydrolysis and biochemical methane potential of secondary sludge Author Kim, Sangmin (Pohang University of Science and Technology) Woo, Seung Gyun (Korea Research Institute of Bioscience and Biotechnology) Lee, J. (TU Delft Sanitary Engineering) Lee, Dae Hee (Korea Research Institute of Bioscience and Biotechnology) Hwang, Seokhwan (Pohang University of Science and Technology) Date 2019 Abstract Anaerobic digestion (AD) of secondary sludge is a rate-limiting step due to the bacterial cell wall. In this study, experiments were performed to characterize secondary sludges from three wastewater treatment plants (WWTPs), and to investigate the feasibility of using bacteriophage lysozymes to speed up AD by accelerating the degradation of bacterial cell walls. Protein was the main organic material (67.7% of volatile solids in the sludge). The bacteriophage T4 lysozyme (T4L) was tested for hydrolysis and biochemical methane potential. Variations in the volatile suspended solid (VSS) concentration and biogas production were monitored. The VSS reduction efficiencies by hydrolysis using T4L for 72 h increased and ranged from 17.8% to 26.4%. Biogas production using T4L treated sludges increased and biogas production was increased by as much as 82.4%. Biogas production rate also increased, and the average reaction rate coefficient of first-order kinetics was 0.56 ± 0.02/d, which was up to 47.5% higher compared to the untreated samples at the maximum. Alphaproteobacteria, Betaproteobacteria, Flavobacteriia, Gammaproteobacteria, and Sphingobacteriia were major microbial classes in all sludges. The interpretation of the microbial community structure indicated that T4L treatment is likely to increase the rate of cell wall digestion. Subject Bacterial communityBiochemical methane potential (BMP)Sludge hydrolysis To reference this document use: http://resolver.tudelft.nl/uuid:9c959df1-be62-47bc-a389-6eaa434a9288 DOI https://doi.org/10.3390/en12193644 ISSN 1996-1073 Source Energies, 12 (19) Part of collection Institutional Repository Document type journal article Rights © 2019 Sangmin Kim, Seung Gyun Woo, J. Lee, Dae Hee Lee, Seokhwan Hwang Files PDF energies_12_03644_v2.pdf 1.18 MB Close viewer /islandora/object/uuid:9c959df1-be62-47bc-a389-6eaa434a9288/datastream/OBJ/view