Rhodococcus strains as source for ene-reductase activity

Rhodococcus strains as source for ene-reductase activity

Chen, B. (TU Delft BT/Biocatalysis; Sun Yat-sen University)
Medici, R. (TU Delft BT/Biocatalysis)
van der Helm, M. (TU Delft BT/Biocatalysis)
van Zwet, Y.A.A. (TU Delft BT/Biocatalysis)
Gjonaj, L. (TU Delft BT/Biocatalysis; Leiden University Medical Center)
van der Geest, R.C.S. (TU Delft BT/Biocatalysis)
Otten, L.G. (TU Delft BT/Biocatalysis)
Hanefeld, U. (TU Delft BT/Biocatalysis)

2018

Rhodococcus strains are ubiquitous in nature and known to metabolise a wide variety of compounds. At the same time, asymmetric reduction of C=C bonds is important in the production of high-valued chiral building blocks. In order to evaluate if Rhodococci can be used for this task, we have probed several Rhodococcus rhodochrous and R. erythropolis strains for ene-reductase activity. A series of substrates including activated ketones, an aldehyde, an imide and nitro-compound were screened using whole cells of seven Rhodococcus strains. This revealed that whole cells of all Rhodococcus strains showed apparent (S)-selectivity towards ketoisophorone, while most other organisms show (R)-selectivity for this compound. Three putative ene-reductases from R. rhodochrous ATCC 17895 were heterologously expressed in Escherichia coli. One protein was purified and its biocatalytic and biochemical properties were characterised, showing typical (enantioselective) properties for class 3 ene-reductases of the old yellow enzyme family.

Asymmetric reduction
Enantioselectivity
Ene-reductase
Rhodococcus

http://resolver.tudelft.nl/uuid:b7b0f93f-4b18-4bf5-82ec-fa7ea0c38d67

https://doi.org/10.1007/s00253-018-8984-7

0175-7598

Applied Microbiology and Biotechnology, 1-12

Institutional Repository

journal article

© 2018 B. Chen, R. Medici, M. van der Helm, Y.A.A. van Zwet, L. Gjonaj, R.C.S. van der Geest, L.G. Otten, U. Hanefeld