Print Email Facebook Twitter RNA sequencing data integration reveals an miRNA interactome of osteoarthritis cartilage Title RNA sequencing data integration reveals an miRNA interactome of osteoarthritis cartilage Author Coutinho de Almeida, Rodrigo (Leiden University Medical Center) Ramos, Yolande F.M. (Leiden University Medical Center) Mahfouz, A.M.E.T.A. (TU Delft Pattern Recognition and Bioinformatics; Leiden University Medical Center) den Hollander, Wouter (Leiden University Medical Center) Lakenberg, Nico (Leiden University Medical Center) Houtman, Evelyn (Leiden University Medical Center) van Hoolwerff, Marcella (Leiden University Medical Center) Suchiman, H. Eka D. (Leiden University Medical Center) Rodriguez-Ruiz, Alejandro (Leiden University Medical Center) Slagboom, P. Eline (Leiden University Medical Center) Mei, Hailiang (Leiden University Medical Center) Kielbasa, Szymon M. (Leiden University Medical Center) Nelissen, R.G.H.H. (Leiden University Medical Center) Reinders, M.J.T. (TU Delft Pattern Recognition and Bioinformatics; Leiden University Medical Center) Meulenbelt, Ingrid (Leiden University Medical Center) Date 2018 Abstract Objective To uncover the microRNA (miRNA) interactome of the osteoarthritis (OA) pathophysiological process in the cartilage.Methods We performed RNA sequencing in 130 samples (n=35 and n=30 pairs for messenger RNA (mRNA) and miRNA, respectively) on macroscopically preserved and lesioned OA cartilage from the same patient and performed differential expression (DE) analysis of miRNA and mRNAs. To build an OA-specific miRNA interactome, a prioritisation scheme was applied based on inverse Pearson’s correlations and inverse DE of miRNAs and mRNAs. Subsequently, these were filtered by those present in predicted (TargetScan/microT-CDS) and/or experimentally validated (miRTarBase/TarBase) public databases. Pathway enrichment analysis was applied to elucidate OA-related pathways likely mediated by miRNA regulatory mechanisms.Results We found 142 miRNAs and 2387 mRNAs to be differentially expressed between lesioned and preserved OA articular cartilage. After applying prioritisation towards likely miRNA-mRNA targets, a regulatory network of 62 miRNAs targeting 238 mRNAs was created. Subsequent pathway enrichment analysis of these mRNAs (or genes) elucidated that genes within the ‘nervous system development’ are likely mediated by miRNA regulatory mechanisms (familywise error=8.4×10−5). Herein NTF3 encodes neurotrophin-3, which controls survival and differentiation of neurons and which is closely related to the nerve growth factor.Conclusions By an integrated approach of miRNA and mRNA sequencing data of OA cartilage, an OA miRNA interactome and related pathways were elucidated. Our functional data demonstrated interacting levels at which miRNA affects expression of genes in the cartilage and exemplified the complexity of functionally validating a network of genes that may be targeted by multiple miRNAs. To reference this document use: http://resolver.tudelft.nl/uuid:c14002d2-02be-42a7-b1ba-52b8155e194e DOI https://doi.org/10.1136/annrheumdis-2018-213882 ISSN 0003-4967 Source Annals of the Rheumatic Diseases: an international peer-reviewed journal for health professionals and researchers in the rheumatic diseases, 1-8 Part of collection Institutional Repository Document type journal article Rights © 2018 Rodrigo Coutinho de Almeida, Yolande F.M. Ramos, A.M.E.T.A. Mahfouz, Wouter den Hollander, Nico Lakenberg, Evelyn Houtman, Marcella van Hoolwerff, H. Eka D. Suchiman, Alejandro Rodriguez-Ruiz, P. Eline Slagboom, Hailiang Mei, Szymon M. Kielbasa, R.G.H.H. Nelissen, M.J.T. Reinders, Ingrid Meulenbelt Files PDF 47774646_annrheumdis_2018 ... 2.full.pdf 1.09 MB Close viewer /islandora/object/uuid:c14002d2-02be-42a7-b1ba-52b8155e194e/datastream/OBJ/view