Genome-wide effect of non-optimal temperatures under anaerobic conditions on gene expression in Saccharomyces cerevisiae

Genome-wide effect of non-optimal temperatures under anaerobic conditions on gene expression in Saccharomyces cerevisiae

García-Ríos, Estéfani (Institute of Agrochemistry and Food Technology; University of Valencia)
Alonso-del-Real, Javier (Institute of Agrochemistry and Food Technology)
Lip, K.Y.F. (TU Delft BT/Biotechnology and Society)
Pinheiro, Tania (University of Minho)
Teixeira, José (University of Minho)
van Gulik, W.M. (TU Delft BT/Industriele Microbiologie)
Domingues, Lucília (University of Minho)
Querol, Amparo (Institute of Agrochemistry and Food Technology)
Guillamón, José Manuel (Institute of Agrochemistry and Food Technology)

2022

Understanding of thermal adaptation mechanisms in yeast is crucial to develop better-adapted strains to industrial processes, providing more economical and sustainable products. We have analyzed the transcriptomic responses of three Saccharomyces cerevisiae strains, a commercial wine strain, ADY5, a laboratory strain, CEN.PK113-7D and a commercial bioethanol strain, Ethanol Red, grown at non-optimal temperatures under anaerobic chemostat conditions. Transcriptomic analysis of the three strains revealed a huge complexity of cellular mechanisms and responses. Overall, cold exerted a stronger transcriptional response in the three strains comparing with heat conditions, with a higher number of down-regulating genes than of up-regulating genes regardless the strain analyzed. The comparison of the transcriptome at both sub- and supra-optimal temperatures showed the presence of common genes up- or down-regulated in both conditions, but also the presence of common genes up- or down-regulated in the three studied strains. More specifically, we have identified and validated three up-regulated genes at sub-optimal temperature in the three strains, OPI3, EFM6 and YOL014W. Finally, the comparison of the transcriptomic data with a previous proteomic study with the same strains revealed a good correlation between gene activity and protein abundance, mainly at low temperature. Our work provides a global insight into the specific mechanisms involved in temperature adaptation regarding both transcriptome and proteome, which can be a step forward in the comprehension and improvement of yeast thermotolerance.

Cold
Heat
Proteome
Saccharomyces
Temperature tolerance
Transcriptome

http://resolver.tudelft.nl/uuid:cf56b331-1f87-461c-955e-9aed132d0b60

https://doi.org/10.1016/j.ygeno.2022.110386

0888-7543

Genomics, 114 (4)

Institutional Repository

journal article

© 2022 Estéfani García-Ríos, Javier Alonso-del-Real, K.Y.F. Lip, Tania Pinheiro, José Teixeira, W.M. van Gulik, Lucília Domingues, Amparo Querol, José Manuel Guillamón