Stable Free-Standing Lipid Bilayer Membranes in Norland Optical Adhesive 81 Microchannels

Stable Free-Standing Lipid Bilayer Membranes in Norland Optical Adhesive 81 Microchannels

Marin, Victor (Kavli institute of nanoscience Delft)
Kieffer, R.Y. (TU Delft BN/Marie-Eve Aubin-Tam Lab; Kavli institute of nanoscience Delft)
Padmos, Raymond (Kavli institute of nanoscience Delft)
Aubin-Tam, M.E. (TU Delft BN/Marie-Eve Aubin-Tam Lab; Kavli institute of nanoscience Delft)

2016-06-28

We report a simple, cost-effective, and reproducible method to form free-standing lipid bilayer membranes in microdevices made with Norland Optical Adhesive 81 (NOA81). Surface treatment with either alkylsilane or fluoroalkylsilane enables the self-assembly of stable 1,2-diphytanoyl-sn-glycero-3-phosphocholine 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)/1,2-dihexadecanoyl-sn-glycero-3-phosphocholine (DPPC) membranes. Capacitance measurements are used to characterize the lipid bilayer and to follow its formation in real-time. With current recordings, we detect the insertion of single α-hemolysin pores into the bilayer membrane, demonstrating the possibility of using this device for single-channel electrophysiology sensing applications. Optical transparency of the device and vertical position of the lipid bilayer with respect to the microscope focal plane allows easy integration with other single-molecule techniques, such as optical tweezers. Therefore, this method to form long-lived lipid bilayers finds a wide range of applications, from sensing measurements to biophysical studies of lipid bilayers and associated proteins.

http://resolver.tudelft.nl/uuid:d32e2058-9ef7-4b4c-949d-4fe6a2a3c662

https://doi.org/10.1021/acs.analchem.6b00926

2017-06-28

0003-2700

Analytical Chemistry, 88 (15), 7466-7470

Accepted Author Manuscript

Institutional Repository

journal article

© 2016 Victor Marin, R.Y. Kieffer, Raymond Padmos, M.E. Aubin-Tam