Print Email Facebook Twitter Extreme calorie restriction and energy source starvation in Saccharomyces cerevisiae represent distinct physiological states Title Extreme calorie restriction and energy source starvation in Saccharomyces cerevisiae represent distinct physiological states Author Boender, L.G.M. Almering, M.J.H. Dijk, M. Van Maris, A.J.A. De Winde, J.H. Pronk, J.T. Daran-Lapujade, P. Faculty Applied Sciences Department BT/Biotechnology Date 2011-07-22 Abstract Cultivation methods used to investigate microbial calorie restriction often result in carbon and energy starvation. This study aims to dissect cellular responses to calorie restriction and starvation in Saccharomyces cerevisiae by using retentostat cultivation. In retentostats, cells are continuously supplied with a small, constant carbon and energy supply, sufficient for maintenance of cellular viability and integrity but insufficient for growth. When glucose-limited retentostats cultivated under extreme calorie restriction were subjected to glucose starvation, calorie-restricted and glucose-starved cells were found to share characteristics such as increased heat-shock tolerance and expression of quiescence-related genes. However, they also displayed strikingly different features. While calorie-restricted yeast cultures remained metabolically active and viable for prolonged periods of time, glucose starvation resulted in rapid consumption of reserve carbohydrates, population heterogeneity due to appearance of senescent cells and, ultimately, loss of viability. Moreover, during starvation, calculated rates of ATP synthesis from reserve carbohydrates were 2–3 orders of magnitude lower than steady-state ATP-turnover rates calculated under extreme calorie restriction in retentostats. Stringent reduction of ATP turnover during glucose starvation was accompanied by a strong down-regulation of genes involved in protein synthesis. These results demonstrate that extreme calorie restriction and carbon starvation represent different physiological states in S. cerevisiae. Subject retentostatsaccharomyces cerevisiaecalorie restrictioncarbon starvationreserve carbohydratespopulation heterogeneityOA-Fund TU Delft To reference this document use: http://resolver.tudelft.nl/uuid:d3547b05-e991-4092-9ce5-43afdbe283cc DOI https://doi.org/10.1016/j.bbamcr.2011.07.008 Publisher Elsevier ISSN 0006-3002 Source Biochimica et Biophysica Acta. Molecular Cell Research, 1813 (12), 2011 Part of collection Institutional Repository Document type journal article Rights (c) 2011 ElsevierThe Author(s) Files PDF A9R939A.pdf 832.81 KB Close viewer /islandora/object/uuid:d3547b05-e991-4092-9ce5-43afdbe283cc/datastream/OBJ/view