Print Email Facebook Twitter High-resolution reconstruction of a Jumbo-bacteriophage infecting capsulated bacteria using hyperbranched tail fibers Title High-resolution reconstruction of a Jumbo-bacteriophage infecting capsulated bacteria using hyperbranched tail fibers Author Ouyang, Ruochen (Xi’an Jiaotong University; Universiteit Leiden) Martins Costa, A.R. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft) Cassidy, C. Keith (University of Oxford) Otwinowska, Aleksandra (University of Wroclaw) Williams, Vera C.J. (Universiteit Leiden) Latka, Agnieszka (University of Wroclaw; Universiteit Gent) Stansfeld, Phill J. (University of Warwick) Drulis-Kawa, Zuzanna (University of Wroclaw) Brouns, S.J.J. (TU Delft BN/Bionanoscience; TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft) Department BN/Bionanoscience Date 2022 Abstract The Klebsiella jumbo myophage ϕKp24 displays an unusually complex arrangement of tail fibers interacting with a host cell. In this study, we combine cryo-electron microscopy methods, protein structure prediction methods, molecular simulations, microbiological and machine learning approaches to explore the capsid, tail, and tail fibers of ϕKp24. We determine the structure of the capsid and tail at 4.1 Å and 3.0 Å resolution. We observe the tail fibers are branched and rearranged dramatically upon cell surface attachment. This complex configuration involves fourteen putative tail fibers with depolymerase activity that provide ϕKp24 with the ability to infect a broad panel of capsular polysaccharide (CPS) types of Klebsiella pneumoniae. Our study provides structural and functional insight into how ϕKp24 adapts to the variable surfaces of capsulated bacterial pathogens, which is useful for the development of phage therapy approaches against pan-drug resistant K. pneumoniae strains. To reference this document use: http://resolver.tudelft.nl/uuid:d3f653d1-c3dc-4d28-b9ea-30cceef89e87 DOI https://doi.org/10.1038/s41467-022-34972-5 ISSN 2041-1723 Source Nature Communications, 13 (1), 16 Part of collection Institutional Repository Document type journal article Rights © 2022 Ruochen Ouyang, A.R. Martins Costa, C. Keith Cassidy, Aleksandra Otwinowska, Vera C.J. Williams, Agnieszka Latka, Phill J. Stansfeld, Zuzanna Drulis-Kawa, S.J.J. Brouns, More Authors Files PDF s41467_022_34972_5.pdf 10.95 MB Close viewer /islandora/object/uuid:d3f653d1-c3dc-4d28-b9ea-30cceef89e87/datastream/OBJ/view