Print Email Facebook Twitter The acid soluble extracellular polymeric substance of aerobic granular sludge dominated by Defluviicoccus sp. Title The acid soluble extracellular polymeric substance of aerobic granular sludge dominated by Defluviicoccus sp. Author Pronk, M. (TU Delft BT/Environmental Biotechnology) Neu, Thomas R. (Helmholtz Centre for Environmental Research - UFZ) van Loosdrecht, Mark C.M. (TU Delft BT/Environmental Biotechnology) Lin, Y. (TU Delft BT/Environmental Biotechnology) Date 2017-05-30 Abstract A new acid soluble extracellular polymeric substance (acid soluble EPS) was extracted from an acetate fed aerobic granular sludge reactor operated at 35 °C. Acid soluble EPS dominated granules exhibited a remarkable and distinctive tangled tubular morphology. These granules are dominated by Defluviicoccus Cluster II organisms. Acetic acid instead of the usually required alkaline extraction medium was needed to dissolve the granules and solubilise the polymeric matrix. The extracted acid soluble EPS was analysed and identified using various instrumental analysis including 1H and 13C Nuclear Magnetic Resonance, Fourier Transform Infrared Spectroscopy and Raman spectroscopy. In addition, the glycoconjugates were characterized by fluorescence lectin-binding analysis. The acid soluble EPS is α-(1 → 4) linked polysaccharide, containing both glucose and galactose as monomers. There are –OCH3 groups connected to the glucose monomer. Transmission and scanning electron microscopy (TEM, SEM) as well as confocal laser scanning microscopy (CLSM) showed that the acid soluble EPS was present as a tightly bound capsular EPS around bacterial cells ordered into a sarcinae-like growth pattern. The special granule morphology is decided by the acid soluble EPS produced by Defluviicoccus Cluster II organisms. This work shows that no single one method can be used to extract all possible extracellular polymeric substances. Results obtained here can support the elucidation of biofilm formation and structure in future research. Subject Aerobic granular sludgeBiofilmDefluviicoccusEPS extractionExtracellular polymeric substances To reference this document use: http://resolver.tudelft.nl/uuid:da0155b8-20b3-47e1-9559-f03da8d0ace8 DOI https://doi.org/10.1016/j.watres.2017.05.068 ISSN 0043-1354 Source Water Research, 122, 148-158 Part of collection Institutional Repository Document type journal article Rights © 2017 M. Pronk, Thomas R. Neu, Mark C.M. van Loosdrecht, Y. Lin Files PDF 1_s2.0_S0043135417304323_main.pdf 4.04 MB Close viewer /islandora/object/uuid:da0155b8-20b3-47e1-9559-f03da8d0ace8/datastream/OBJ/view