Print Email Facebook Twitter Cardiolipin enhances the enzymatic activity of cytochrome bd and cytochrome bo 3 solubilized in dodecyl-maltoside Title Cardiolipin enhances the enzymatic activity of cytochrome bd and cytochrome bo 3 solubilized in dodecyl-maltoside Author Asseri, Amer H. (King Abdulaziz University; Vrije Universiteit Amsterdam) Godoy Hernandez, A. (TU Delft BT/Biocatalysis) Goojani, Hojjat Ghasemi (Vrije Universiteit Amsterdam) Lill, Holger (Vrije Universiteit Amsterdam) Sakamoto, Junshi (Kyushu Institute of Technology) McMillan, D.G.G. (TU Delft BT/Biocatalysis) Bald, Dirk (Vrije Universiteit Amsterdam) Date 2021 Abstract Cardiolipin (CL) is a lipid that is found in the membranes of bacteria and the inner membranes of mitochondria. CL can increase the activity of integral membrane proteins, in particular components of respiratory pathways. We here report that CL activated detergent-solubilized cytochrome bd, a terminal oxidase from Escherichia coli. CL enhanced the oxygen consumption activity ~ twofold and decreased the apparent KM value for ubiquinol-1 as substrate from 95 µM to 35 µM. Activation by CL was also observed for cytochrome bd from two Gram-positive species, Geobacillus thermodenitrificans and Corynebacterium glutamicum, and for cytochrome bo3 from E. coli. Taken together, CL can enhance the activity of detergent-solubilized cytochrome bd and cytochrome bo3. To reference this document use: http://resolver.tudelft.nl/uuid:eda08571-468d-4b7c-a862-c442320d8a8b DOI https://doi.org/10.1038/s41598-021-87354-0 ISSN 2045-2322 Source Scientific Reports, 11 (1) Part of collection Institutional Repository Document type journal article Rights © 2021 Amer H. Asseri, A. Godoy Hernandez, Hojjat Ghasemi Goojani, Holger Lill, Junshi Sakamoto, D.G.G. McMillan, Dirk Bald Files PDF s41598_021_87354_0.pdf 1.51 MB Close viewer /islandora/object/uuid:eda08571-468d-4b7c-a862-c442320d8a8b/datastream/OBJ/view