Print Email Facebook Twitter Functionality-packed additively manufactured porous titanium implants Title Functionality-packed additively manufactured porous titanium implants Author van Hengel, I.A.J. (TU Delft Biomaterials & Tissue Biomechanics) Gelderman, F. S.A. (Student TU Delft) Athanasiadis, S. (TU Delft Mechanical Engineering) Minneboo, M.B. (TU Delft Biomaterials & Tissue Biomechanics) Weinans, Harrie (TU Delft Biomaterials & Tissue Biomechanics; University Medical Center Utrecht) Fluit, A. C. (University Medical Center Utrecht) van der Eerden, B.C.J. (Erasmus MC) Fratila-Apachitei, E.L. (TU Delft Biomaterials & Tissue Biomechanics) Apachitei, I. (TU Delft Biomaterials & Tissue Biomechanics) Zadpoor, A.A. (TU Delft Biomaterials & Tissue Biomechanics) Faculty Mechanical Engineering Date 2020 Abstract The holy grail of orthopedic implant design is to ward off both aseptic and septic loosening for long enough that the implant outlives the patient. Questing this holy grail is feasible only if orthopedic biomaterials possess a long list of functionalities that enable them to discharge the onerous task of permanently replacing the native bone tissue. Here, we present a rationally designed and additive manufacturing (AM) topologically ordered porous metallic biomaterial that is made from Ti-6Al-4V using selective laser melting and packs most (if not all) of the required functionalities into a single implant. In addition to presenting a fully interconnected porous structure and form-freedom that enables realization of patient-specific implants, the biomaterials developed here were biofunctionalized using plasma electrolytic oxidation to locally release both osteogenic (i.e. strontium) and antibacterial (i.e. silver ions) agents. The same single-step biofunctionalization process also incorporated hydroxyapatite into the surface of the implants. Our measurements verified the continued release of both types of active agents up to 28 days. Assessment of the antibacterial activity in vitro and in an ex vivo murine model demonstrated extraordinarily high levels of bactericidal effects against a highly virulent and multidrug-resistant Staphylococcus aureus strain (i.e. USA300) with total eradication of both planktonic and adherent bacteria. This strong antibacterial behavior was combined with a significantly enhanced osteogenic behavior, as evidenced by significantly higher levels of alkaline phosphatase (ALP) activity compared with non-biofunctionalized implants. Finally, we discovered synergistic antibacterial behavior between strontium and silver ions, meaning that 4–32 folds lower concentrations of silver ions were required to achieve growth inhibition and total killing of bacteria. The functionality-packed biomaterial presented here demonstrates a unique combination of functionalities that make it an advanced prototype of future orthopedic biomaterials where implants will outlive patients. Subject Additive manufacturingAntimicrobial implantBiofunctionalizationMultifunctional surfacesSilver nanoparticlesStrontium To reference this document use: http://resolver.tudelft.nl/uuid:eedc3866-54e8-4b26-b4d7-4dc6ba792ca3 DOI https://doi.org/10.1016/j.mtbio.2020.100060 ISSN 2590-0064 Source Materials Today Bio, 7 Part of collection Institutional Repository Document type journal article Rights © 2020 I.A.J. van Hengel, F. S.A. Gelderman, S. Athanasiadis, M.B. Minneboo, Harrie Weinans, A. C. Fluit, B.C.J. van der Eerden, E.L. Fratila-Apachitei, I. Apachitei, A.A. Zadpoor Files PDF 1_s2.0_S259000642030020X_main.pdf 4.84 MB Close viewer /islandora/object/uuid:eedc3866-54e8-4b26-b4d7-4dc6ba792ca3/datastream/OBJ/view