Print Email Facebook Twitter Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to non-optimal temperatures under anaerobic conditions Title Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to non-optimal temperatures under anaerobic conditions Author Pinheiro, Tânia (University of Minho) Lip, K.Y.F. (TU Delft BT/Biotechnology and Society) García-Ríos, Estéfani (Institute of Agrochemistry and Food Technology) Querol, Amparo (University of the Balearic Islands) Teixeira, José (University of Minho) van Gulik, W.M. (TU Delft BT/Industriele Microbiologie) Guillamón, José Manuel (University of the Balearic Islands) Domingues, Lucília (University of Minho) Date 2020 Abstract Elucidation of temperature tolerance mechanisms in yeast is essential for enhancing cellular robustness of strains, providing more economically and sustainable processes. We investigated the differential responses of three distinct Saccharomyces cerevisiae strains, an industrial wine strain, ADY5, a laboratory strain, CEN.PK113-7D and an industrial bioethanol strain, Ethanol Red, grown at sub- and supra-optimal temperatures under chemostat conditions. We employed anaerobic conditions, mimicking the industrial processes. The proteomic profile of these strains in all conditions was performed by sequential window acquisition of all theoretical spectra-mass spectrometry (SWATH-MS), allowing the quantification of 997 proteins, data available via ProteomeXchange (PXD016567). Our analysis demonstrated that temperature responses differ between the strains; however, we also found some common responsive proteins, revealing that the response to temperature involves general stress and specific mechanisms. Overall, sub-optimal temperature conditions involved a higher remodeling of the proteome. The proteomic data evidenced that the cold response involves strong repression of translation-related proteins as well as induction of amino acid metabolism, together with components related to protein folding and degradation while, the high temperature response mainly recruits amino acid metabolism. Our study provides a global and thorough insight into how growth temperature affects the yeast proteome, which can be a step forward in the comprehension and improvement of yeast thermotolerance. To reference this document use: http://resolver.tudelft.nl/uuid:fb091597-381e-4921-9e31-5b02a6615766 DOI https://doi.org/10.1038/s41598-020-77846-w ISSN 2045-2322 Source Scientific Reports, 10 (1) Part of collection Institutional Repository Document type journal article Rights © 2020 Tânia Pinheiro, K.Y.F. Lip, Estéfani García-Ríos, Amparo Querol, José Teixeira, W.M. van Gulik, José Manuel Guillamón, Lucília Domingues Files PDF s41598_020_77846_w.pdf 2.19 MB Close viewer /islandora/object/uuid:fb091597-381e-4921-9e31-5b02a6615766/datastream/OBJ/view