Print Email Facebook Twitter Fibre formation in calcium caseinate influenced by solvent isotope effect and drying method – A neutron spectroscopy study Title Fibre formation in calcium caseinate influenced by solvent isotope effect and drying method – A neutron spectroscopy study Author Tian, B. (TU Delft RST/Neutron and Positron Methods in Materials) Garcia Sakai, Victoria (Rutherford Appleton Laboratory) Pappas, C. (TU Delft RST/Neutron and Positron Methods in Materials) van der Goot, Atze Jan (Wageningen University & Research) Bouwman, W.G. (TU Delft RST/Neutron and Positron Methods in Materials) Date 2019 Abstract We present an investigation of the dynamics of calcium caseinate as a function of hydration, solvent isotope (H2O and D2O) and drying methods (roller drying and spray drying), using quasi-elastic neutron scattering (QENS). These factors are key to the formation of fibres in this material which makes it a potential candidate as a next-generation meat analogue. Using a phenomenological model, we find that the relaxation times of the dry spray dried powder decrease with increasing temperatures, while they do not change for the roller dried powder. The spectra of the hydrated samples reveal two independent picosecond processes, both reflecting localized re-orientational motions. We hypothesize that the faster motion is due to the external protein groups that are hydrophilic and the slower motion is due to the internal groups that are hydrophobic. The solvent effect of D2O is not limited to the external groups but prevails to the internal groups where less protons are mobile compared to the H2O hydrated samples. Higher temperatures narrow the number difference in mobile protons, possibly by altering the weak interactions inside the protein aggregates. These findings suggest that a harsh and longer drying process contributes to less active protein side-groups and highlight the hydrophobic effect of D2O on the fibre formation in calcium caseinate. Subject Calcium caseinateDrying methodFibre formationNeutron spectroscopyProtein dynamicsSolvent isotope effect To reference this document use: http://resolver.tudelft.nl/uuid:03159d0d-e0b0-4c5a-8e56-1c44503431e9 DOI https://doi.org/10.1016/j.ces.2019.07.023 Embargo date 2020-05-02 ISSN 0009-2509 Source Chemical Engineering Science, 207, 1270-1277 Bibliographical note Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public. Part of collection Institutional Repository Document type journal article Rights © 2019 B. Tian, Victoria Garcia Sakai, C. Pappas, Atze Jan van der Goot, W.G. Bouwman Files PDF 1_s2.0_S000925091930586X_ ... averne.pdf 1.44 MB Close viewer /islandora/object/uuid:03159d0d-e0b0-4c5a-8e56-1c44503431e9/datastream/OBJ/view