Title
Clinical, mechanical, and immunohistopathological effects of tissue adhesives on the colon: An in-vivo study
Author
Vakalopoulos, Konstantinos A. (Erasmus Medical Center)
Wu, Zhouqiao (Erasmus Medical Center)
Kroese, Leonard F. (Erasmus Medical Center)
van der Horst, Paul H. (Erasmus Medical Center)
Lam, King H. (Erasmus Medical Center)
Dodou, D. (TU Delft Medical Instruments & Bio-Inspired Technology) 
Jeekel, Johannes (Erasmus Medical Center)
Lange, Johan F. (Erasmus Medical Center)
Date
2017
Abstract
Background: Tissue adhesives may be useful for sealing bowel anastomoses by preventing anastomotic leakage. Prior to clinical implementation, an in-depth analysis of the clinical and immunohistopathological effects of tissue adhesives on the target tissue and of the mechanical strength of the adhesive bond in an in vivo model is needed. Materials and methods: In 84 rats, two bowel segments were glued using one of the following tissue adhesive: Bioglue, Gelatin-resorcinol-formaldehyde (GRF), Glubran 2, Histoacryl Flex, Omnex, Duraseal Xact, or Tissucol. Rats were followed for 7 or 28 days. Endpoints were clinical complication rate, mechanical strength, and immunohistopathological reactions. Results: Of the seven tissue adhesives, GRF and Bioglue showed the highest rates of bowel wall destruction and ileus and the most severe immunohistopathological tissue reactions at 7 and 28 days. Cyanoacrylates (Histoacryl Flex, Omnex, Glubran 2) showed high mechanical strength and mild immunohistopathological reactions at 7 and 28 days. Duraseal Xact and Tissucol were the most inert tissue adhesives, but exhibited low mechanical strength. At 28 days, mechanical strength was significantly correlated to CD8, CD68, and Ki67 cell counts. Conclusion: Based on the clinical and immunohistopathological outcomes, GRF and Bioglue were found to be the least suitable tissue adhesives for colonic use. Duraseal Xact and Tissucol were inert but also showed low mechanical strength. Cyanoacrylates exhibited mild clinical and immunohistopathological effects while maintaining high strength, which makes them promising as colonic sealants.
Subject
colorectal
complications
gastrointestinal
surgery
tissue adhesive
tissue glue
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http://resolver.tudelft.nl/uuid:18d49f5d-74f6-40ae-9c19-85489d6c8872
DOI
https://doi.org/10.1002/jbm.b.33621
Embargo date
2017-01-24
ISSN
1552-4973
Source
Journal of Biomedical Materials Research. Part B: Applied Biomaterials, 105 (4), 846-854
Bibliographical note
Accepted Author Manuscript
Part of collection
Institutional Repository
Document type
journal article
Rights
© 2017 Konstantinos A. Vakalopoulos, Zhouqiao Wu, Leonard F. Kroese, Paul H. van der Horst, King H. Lam, D. Dodou, Johannes Jeekel, Johan F. Lange