The gRAMP CRISPR-Cas effector is an RNA endonuclease complexed with a caspase-like peptidase

van Beljouw, S.P.B.; van Eijkeren-Haagsma, A.C.; Rodriguez Molina, A.; van den Berg, D.F.; Vink, J.N.A.; Brouns, S.J.J.

doi:10.1126/science.abk2718

The gRAMP CRISPR-Cas effector is an RNA endonuclease complexed with a caspase-like peptidase

Title

The gRAMP CRISPR-Cas effector is an RNA endonuclease complexed with a caspase-like peptidase

Author

van Beljouw, S.P.B. (TU Delft BN/Stan Brouns Lab)
van Eijkeren-Haagsma, A.C. (TU Delft BN/Technici en Analisten; Kavli institute of nanoscience Delft)
Rodriguez Molina, A. (TU Delft BT/Biocatalysis; Kavli institute of nanoscience Delft)
van den Berg, D.F. (External organisation; Kavli institute of nanoscience Delft)
Vink, J.N.A. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft)
Brouns, S.J.J. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft)

Date

2021

Abstract

Type III CRISPR-Cas immunity is widespread in prokaryotes and is generally mediated by multisubunit effector complexes. These complexes recognize complementary viral transcripts and can activate ancillary immune proteins. Here, we describe a type III-E effector from Candidatus “Scalindua brodae” (Sb-gRAMP), which is natively encoded by a single gene with several type III domains fused together. This effector uses CRISPR RNA to guide target RNA recognition and cleaves single-stranded RNA at two defined positions six nucleotides apart. Sb-gRAMP physically combines with the caspase-like TPR-CHAT peptidase to form the CRISPR-guided caspase (Craspase) complex, suggesting a potential mechanism of target RNA-induced protease activity to gain viral immunity.

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DOI

https://doi.org/10.1126/science.abk2718

ISSN

0036-8075

Source

Science (New York, N.Y.), 373 (6561), 1349-1353

Bibliographical note

Accepted Author Manuscript

Part of collection

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