Title
Biological applications of an LCoS-BASED PROGRAMMABLE ARRAY MICROSCOPE (PAM)
Author
Hagen, G.M.
Caarls, W.
Thomas, M.
Hill, A.
Lidke, K.A.
Rieger, B.
Fritsch, C.
Van Geest, B.
Jovin, T.M.
Arndt-Jovin, D.J.
Faculty
Electrical Engineering, Mathematics and Computer Science
Department
Quantitative Imaging Group
Date
2007-02-13
Abstract
We report on a new generation, commercial prototype of a programmable array optical sectioning fluorescence microscope (PAM) for rapid, light efficient 3D imaging of living specimens. The stand-alone module, including light source(s) and detector(s), features an innovative optical design and a ferroelectric liquid-crystal-on-silicon (LCoS) spatial light modulator (SLM) instead of the DMD used in the original PAM design. The LCoS PAM (developed in collaboration with Cairn Research, Ltd.) can be attached to a port of a(ny) unmodified fluorescence microscope. The prototype system currently operated at the Max Planck Institute incorporates a 6-position high-intensity LED illuminator, modulated laser and lamp light sources, and an Andor iXon emCCD camera. The module is mounted on an Olympus IX71 inverted microscope with 60-150X objectives with a Prior Scientific x,y, and z high resolution scanning stages. Further enhancements recently include: (i) point- and line-wise spectral resolution and (ii) lifetime imaging (FLIM) in the frequency domain. Multiphoton operation and other nonlinear techniques should be feasible. The capabilities of the PAM are illustrated by several examples demonstrating single molecule as well as lifetime imaging in live cells, and the unique capability to perform photoconversion with arbitrary patterns and high spatial resolution. Using quantum dot coupled ligands we show real-time binding and subsequent trafficking of individual ligand-growth factor receptor complexes on and in live cells with a temporal resolution and sensitivity exceeding those of conventional CLSM systems. The combined use of a blue laser and parallel LED or visible laser sources permits photoactivation and rapid kinetic analysis of cellular processes probed by photoswitchable visible fluorescent proteins such as DRONPA.
Subject
fluorescence microscopy
confocal
emCCD
SLM
quantum dots
EGFR
erbB
DRONPA
FLIM
FRET
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Publisher
SPIE
ISSN
0277-786X
Source
Proceedings of SPIE, 2007 vol. 6441
Part of collection
Institutional Repository
Document type
conference paper
Rights
(c) 2007 Hagen, G.M., Caarls, W., Thomas, M., Hill, A., Lidke, K.A., Rieger, B., Fritsch, C., Van Geest, B., Jovin, T.M., Arndt-Jovin, D.J.