Chemical characterization methods for the analysis of structural extracellular polymeric substances (EPS)

Chemical characterization methods for the analysis of structural extracellular polymeric substances (EPS)

Felz, S. (TU Delft BT/Environmental Biotechnology; TU Delft OLD BT/Cell Systems Engineering)
Vermeulen, Pascalle (Student TU Delft)
van Loosdrecht, Mark C.M. (TU Delft BT/Environmental Biotechnology; TU Delft OLD BT/Cell Systems Engineering)
Lin, Yue Mei (Student TU Delft)

2019

Biofilm structure and functionality depend on extracellular polymeric substances (EPS), but analytical methods for EPS often lack specificity which limits progress of biofilm research. EPS were extracted from aerobic granular sludge and analyzed with frequently applied colorimetric methods. The colorimetric methods were evaluated based on their applicability for EPS analysis. EPS fractions of interest were proteins, sugars, uronic acids and phenolic compounds. The applied methods (Lowry method, bicinchoninic acid assay, phenol sulfuric acid method, carbazole sulfuric acid method) were investigated in terms of their sensitivity towards the selected standard compound. Interference of compounds present in EPS with the colorimetric methods was further evaluated. All methods showed to be highly depending on the choice of standard compound and susceptible towards interference by compounds present in EPS. This study shows that currently used colorimetric methods are not capable of accurately characterizing EPS. More advanced methods are needed to be able to draw conclusions about biofilm composition, structure and functionality.

Aerobic granular sludge
Biofilm
Colorimetric methods
EPS analysis
Extracellular polymeric substances (EPS)

http://resolver.tudelft.nl/uuid:b7b7b4fb-e4ee-474e-a02f-fe214ca68973

https://doi.org/10.1016/j.watres.2019.03.068

0043-1354

Water Research, 157, 201-208

Institutional Repository

journal article

© 2019 S. Felz, Pascalle Vermeulen, Mark C.M. van Loosdrecht, Yue Mei Lin