Engineering ketoreductases for the enantioselective synthesis of chiral alcohols

Journal article (2023)

Authors

Li Qiao Hangzhou Normal University
Zhiyuan Luo Hangzhou Normal University
Haomin Chen Hangzhou Normal University
Pengfei Zhang Hangzhou Normal University
Anming Wang Hangzhou Normal University
R.A. Sheldon BT/Biocatalysis - AS , University of Witwatersrand
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2023 Li Qiao, Zhiyuan Luo, Haomin Chen, Pengfei Zhang, Anming Wang, R.A. Sheldon
DOI: https://doi.org/10.1039/d3cc01474f
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Published Date

2023

Language

English

Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract

The use of engineered ketoreductases (KREDS), both as whole microbial cells and isolated enzymes, in the highly enantiospecific reduction of prochiral ketones is reviewed. The homochiral alcohol products are key intermediates in, for example, pharmaceuticals synthesis. The application of sophisticated protein engineering and enzyme immobilisation techniques to increase industrial viability are discussed.