Searched for:
(1 - 20 of 243)

Pages

document
Kabiri, Y. (author), Angelin, Alessandro (author), Ahmed, Ishtiaq (author), Mutlu, Hatice (author), Bauer, Jens (author), Niemeyer, Christof M. (author), Zandbergen, H.W. (author), Dekker, C. (author)
Staining compounds containing heavy elements (electron dyes) can facilitate the visualization of DNA and related biomolecules by using TEM. However, research into the synthesis and utilization of alternative electron dyes has been limited. Here, we report the synthesis of a novel DNA intercalator molecule, bis-acridine uranyl (BAU). NMR...
journal article 2019
document
Mountain, Christopher (author), Kluba, M.M. (author), Bergers, L.I.J.C. (author), Snijder, Jaap (author), Dekker, R. (author)
Accurate alignment between the cavities in cavity-SOI (c-SOI) wafers and lithography on the wafer surface is essential to advanced MEMS production. Existing alignment methods are well defined, but often require specialized equipment or costly software packages available only in professional manufacturing environments. It would be beneficial...
journal article 2019
document
Quiros Solano, W.F. (author), Gaio, N. (author), Silvestri, C. (author), Pandraud, G. (author), Dekker, R. (author), Sarro, P.M. (author)
Organ-on-chip (OOC) is becoming the alternative tool to conventional in vitro screening. Heart-on-chip devices including microstructures for mechanical and electrical stimulation have been demonstrated to be advantageous to study structural organization and maturation of heart cells. This paper presents the development of metal and polymeric...
journal article 2019
document
Al Nahas, K. (author), Cama, J. (author), Schaich, M. (author), Hammond, K. (author), Deshpande, S.R. (author), Dekker, C. (author), Ryadnov, M. G. (author), Keyser, UF (author)
The spread of bacterial resistance against conventional antibiotics generates a great need for the discovery of novel antimicrobials. Polypeptide antibiotics constitute a promising class of antimicrobial agents that favour attack on bacterial membranes. However, efficient measurement platforms for evaluating their mechanisms of action in a...
journal article 2019
document
Schaich, M. (author), Cama, J. (author), Al Nahas, K. (author), Sobota, Diana (author), Sleath, Hannah (author), Jahnke, Kevin (author), Deshpande, S.R. (author), Dekker, C. (author), Keyser, Ulrich F. (author)
The low membrane permeability of candidate drug molecules is a major challenge in drug development, and insufficient permeability is one reason for the failure of antibiotic treatment against bacteria. Quantifying drug transport across specific pathways in living systems is challenging because one typically lacks knowledge of the exact...
journal article 2019
document
Fanalista, F. (author), Birnie, A.T.F. (author), Maan, R. (author), Burla, Federica (author), Charles usagé Charles-Carty, K.V. (author), Pawlik, G. (author), Deshpande, S.R. (author), Koenderink, Gijsje H. (author), Dogterom, A.M. (author), Dekker, C. (author)
Bottom-up biology is an expanding research field that aims to understand the mechanisms underlying biological processes via in vitro assembly of their essential components in synthetic cells. As encapsulation and controlled manipulation of these elements is a crucial step in the recreation of such cell-like objects, microfluidics is...
journal article 2019
document
Deshpande, S.R. (author), Wunnava Venkata, S.S. (author), Hueting, D.A. (author), Dekker, C. (author)
Recent years have seen a tremendous interest in the bottom-up reconstitution of minimal biomolecular systems, with the ultimate aim of creating an autonomous synthetic cell. One of the universal features of living systems is cell growth, where the cell membrane expands through the incorporation of newly synthesized lipid molecules. Here, the...
journal article 2019
document
Ha, S. (author), Tang, Y. (author), van Oene, M.M. (author), Janissen, R. (author), Dries, R.M. (author), Solano Hermosilla, B.P. (author), Adam, A.J.L. (author), Dekker, N.H. (author)
Optical trapping of (sub)micron-sized particles is broadly employed in nanoscience and engineering. The materials commonly employed for these particles, however, have physical properties that limit the transfer of linear or angular momentum (or both). This reduces the magnitude of forces and torques, and the spatiotemporal resolution,...
journal article 2019
document
Deshpande, S.R. (author), Brandenburg, Frank (author), Lau, A.W.H.R. (author), Last, M.G.F. (author), Spoelstra, W.K. (author), Reese, L. (author), Wunnava, Sreekar (author), Dogterom, A.M. (author), Dekker, C. (author)
Liquid-liquid phase separation (LLPS), especially coacervation, plays a crucial role in cell biology, as it forms numerous membraneless organelles in cells. Coacervates play an indispensable role in regulating intracellular biochemistry, and their dysfunction is associated with several diseases. Understanding of the LLPS dynamics would...
journal article 2019
document
Zhao, Shidi (author), Restrepo Perez, L. (author), Soskine, Misha (author), Maglia, Giovanni (author), Joo, C. (author), Dekker, C. (author), Aksimentiev, A. (author)
Ion channels form the basis of information processing in living cells by facilitating the exchange of electrical signals across and along cellular membranes. Applying the same principles to man-made systems requires the development of synthetic ion channels that can alter their conductance in response to a variety of external manipulations....
journal article 2019
document
Kabiri, Y. (author), Ravelli, Raimond B.G. (author), Lehnert, Tibor (author), Qi, Haoyuan (author), Katan, A.J. (author), Roest, Natascha (author), Kaiser, Ute (author), Dekker, C. (author), Peters, Peter J. (author), Zandbergen, H.W. (author)
Over the last few years, tremendous progress has been made in visualizing biologically important macromolecules using transmission electron microscopy (TEM) and understanding their structure-function relation. Yet, despite the importance of DNA in all forms of life, TEM visualization of individual DNA molecules in its native unlabeled form...
journal article 2019
document
Wu, Fabai (author), Japaridze, A. (author), Zheng, X.Z. (author), Wiktor, J.M. (author), Kerssemakers, J.W.J. (author), Dekker, C. (author)
Although the physical properties of chromosomes, including their morphology, mechanics, and dynamics are crucial for their biological function, many basic questions remain unresolved. Here we directly image the circular chromosome in live E. coli with a broadened cell shape. We find that it exhibits a torus topology with, on average, a lower...
journal article 2019
document
Ordu, O. (author), Lusser, Alexandra (author), Dekker, N.H. (author)
Eukaryotic genomes are hierarchically organized into protein-DNA assemblies for compaction into the nucleus. Nucleosomes, with the (H3-H4)<sub>2</sub> tetrasome as a likely intermediate, are highly dynamic in nature by way of several different mechanisms. We have recently shown that tetrasomes spontaneously change the direction of their DNA...
journal article 2019
document
Mouter, N. (author), Cabral, Manuel Ojeda (author), Dekker, T. (author), van Cranenburgh, Sander (author)
Environmental effects of transport projects have a weak position in Cost-Benefit Analysis (CBA) which might be rooted in the valuation approach adopted in the dominant style of CBA. This conventional valuation approach has been criticized for not valuing positive and negative impacts of transport projects in relation to each other and for not...
journal article 2019
document
Hol, F.J.H. (author), Whitesides, George M. (author), Dekker, C. (author)
Habitat spatial structure has a profound influence on bacterial life, yet there currently are no low-cost equipment-free laboratory techniques to reproduce the intricate structure of natural bacterial habitats. Here, we demonstrate the use of paper scaffolds to create landscapes spatially structured at the scales relevant to bacterial ecology...
journal article 2019
document
Cudejko, Tomasz (author), van der Esch, Martin (author), van den Noort, Josien C. (author), Rijnhart, Judith J.M. (author), van der Leeden, Marike (author), Roorda, Leo D. (author), Lems, Willem (author), Waddington, Gordon (author), Harlaar, J. (author), Dekker, Joost (author)
Objective: To evaluate whether improvement of proprioception, pain, or dynamic knee instability mediates the effect of wearing a soft knee brace on activity limitations in patients with knee osteoarthritis (OA). Methods: We conducted an analysis of data for 44 patients with knee OA who were enrolled in a laboratory-based trial evaluating the...
journal article 2019
document
Dekker, R. (author), van der Meer, F.P. (author), Maljaars, J. (author), Sluys, L.J. (author)
Structures are subjected to cyclic loads that can vary in direction and magnitude, causing constant amplitude mode I simulations to be too simplistic. This study presents a new approach for fatigue crack propagation in ductile materials that can capture mixed-mode loading and overloading. The extended finite element method is used to deal with...
journal article 2019
document
Dekker, W.J.C. (author), Wiersma, S.J. (author), Bouwknegt, J. (author), Pronk, J.T. (author)
In Saccharomyces cerevisiae, acyl-coenzyme A desaturation by Ole1 requires molecular oxygen. Tween 80, a poly-ethoxylated sorbitan-oleate ester, is therefore routinely included in anaerobic growth media as a source of unsaturated fatty acids (UFAs). During optimization of protocols for anaerobic bioreactor cultivation of this yeast, we...
journal article 2019
document
Fragasso, A. (author), Pud, S. (author), Dekker, C. (author)
The performance of solid-state nanopores as promising biosensors is severely hampered by low-frequency 1/f noise in the through-pore ionic current recordings. Here, we develop a model for the 1/f noise in such nanopores, that, unlike previous reports, accounts for contributions from both the pore-cylinder, pore-surface, and access regions. To...
journal article 2019
document
Bracher, J.M. (author), Martinez-Rodriguez, Oscar A. (author), Dekker, W.J.C. (author), Verhoeven, M.D. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)
Expression of a heterologous xylose isomerase, deletion of the GRE3 aldose-reductase gene and overexpression of genes encoding xylulokinase (XKS1) and non-oxidative pentose-phosphate-pathway enzymes (RKI1, RPE1, TAL1, TKL1) enables aerobic growth of Saccharomyces cerevisiae on d-xylose. However, literature reports differ on whether anaerobic...
journal article 2019
Searched for:
(1 - 20 of 243)

Pages