Directing product formation by mixed culture fermentation

Our society is dealing with the shortage of fossil fuels and chemical feedstocks and, on the other hand, with the increase of wastes generated by municipalities, agriculture and industries. Biobased industry, the conversion of renewable resources or wastes to chemicals and fuels, by microbial fermentations or enzymes has been receiving...

Improved p-hydroxybenzoate production by engineered Pseudomonas putida S12 by using a mixed-substrate feeding strategy

The key precursors for p-hydroxybenzoate production by engineered Pseudomonas putida S12 are phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P), for which the pentose phosphate (PP) pathway is an important source. Since PP pathway fluxes are typically low in pseudomonads, E4P and PEP availability is a likely bottleneck for aromatics...

An in vivo analysis of the energetics of aldose oxidation by Acinetobacter calcoaceticus

Xylose anaerobic conversion by open-mixed cultures

Xylose is, after glucose, the dominant sugar in agricultural wastes. In anaerobic environments, carbohydrates are converted into volatile fatty acids and alcohols. These can be used as building blocks in biotechnological or chemical processes, e.g., to produce bioplastics. In this study, xylose fermentation by mixed microbial cultures was...

Effects of growth rate and oxygen tension on glucose dehydrogenase activity in Acinetobacter calcoaceticus LMD 79.41

Heat flux measurements for the fast monitoring of dynamic responses to glucose additions by yeasts that were subjected to different feeding regimes in continuous culture

Diversity of microbial communities in open mixed culture fermentations: Impact of the pH and carbon source

Anaerobic fermentation by an open mixed culture was investigated at different pH values (4–8.5) and with three substrates (glucose, glycerol and xylose). The populations established in each condition were assessed by denaturing gradient gel electrophoresis analysis of the 16S ribosomal RNA gene fragments. The fermentation pattern and the...

Experimental and Theoretical Discrepancies in Growth Yields of Acinetobacter-Calcoaceticus - a Correction of Published Data

Influencing the Insulin System by Placebo Effects in Patients with Diabetes Type 2 and Healthy Controls: A Randomized Controlled Trial

Objective The objective of this study was to investigate whether placebo effect induced by pharmacological conditioning with intranasal insulin can affect glucose, insulin, C-peptide, hunger, and memory in patients with diabetes type 2 and healthy controls. Methods Placebo effect was induced by pharmacological conditioning. Thirty-two older...

Non-enzymatic electrochemical sensing of glucose with nano-structured and functionalised diamond electrodes

Electrochemical sensing is a powerful tool for the rapid detection of (bio)molecules in fluids, and is frequently used in clinical analysis and diagnostics. Diamond is arguably the best electrode material for its robustness, wide potential window, very low background current, biocompatibility and self-cleaning features. <br/>Nowadays, there is a...