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Physiological and Transcriptional Responses of Different Industrial Microbes at Near-Zero Specific Growth Rates

Ercan, O. (author), Bisschops, M.M. (author), Overkamp, W. (author), Jørgensen, T.R. (author), Rama, A.F. (author), Smid, E.J. (author), Pronk, J.T. (author), Kuipers, O.P. (author), Daran-Lapujade, P. (author), Kleerebezem, M. (author)

The current knowledge of the physiology and gene expression of industrially relevant microorganisms is largely based on laboratory studies under conditions of rapid growth and high metabolic activity. However, in natural ecosystems and industrial processes, microbes frequently encounter severe calorie restriction. As a consequence, microbial...

journal article 2015

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Genome-Wide Transcriptional Responses to Carbon Starvation in Nongrowing Lactococcus lactis

Ercan, O. (author), Wels, M. (author), Smid, E.J. (author), Kleerebezem, M. (author)

This paper describes the transcriptional adaptations of nongrowing, retentostat cultures of Lactococcus lactis to starvation. Near-zero-growth cultures (? = 0.0001 h?1) obtained by extended retentostat cultivation were exposed to starvation by termination of the medium supply for 24 h, followed by a recovery period of another 24 h by...

journal article 2015

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Molecular and Metabolic Adaptations of Lactococcus lactis at Near-Zero Growth Rates

Ercan, O. (author), Wels, M. (author), Smid, E.J. (author), Kleerebezem, M. (author)

This paper describes the molecular and metabolic adaptations of Lactococcus lactis during the transition from a growing to a near-zero growth state by using carbon-limited retentostat cultivation. Transcriptomic analyses revealed that metabolic patterns shifted between lactic- and mixed-acid fermentations during retentostat cultivation, which...

journal article 2015

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Transcriptome-Based Characterization of Interactions between Saccharomyces cerevisiae and Lactobacillus delbrueckii subsp. bulgaricus in Lactose-Grown Chemostat Cocultures

Mendes, F. (author), Sieuwerts, S. (author), De Hulster, E. (author), Almering, M.J. (author), Luttik, M.A. (author), Pronk, J.T. (author), Smid, E.J. (author), Bron, P.A. (author), Daran-Lapujade, P. (author)

Mixed populations of Saccharomyces cerevisiae yeasts and lactic acid bacteria occur in many dairy, food, and beverage fermentations, but knowledge about their interactions is incomplete. In the present study, interactions between Saccharomyces cerevisiae and Lactobacillus delbrueckii subsp. bulgaricus, two microorganisms that co-occur in kefir...

journal article 2013

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Understanding the Adaptive Growth Strategy of Lactobacillus plantarum by In Silico Optimisation

Teusink, B. (author), Wiersma, A. (author), Jacobs, L. (author), Notebaart, R.A. (author), Smid, E.J. (author)

In the study of metabolic networks, optimization techniques are often used to predict flux distributions, and hence, metabolic phenotype. Flux balance analysis in particular has been successful in predicting metabolic phenotypes. However, an inherent limitation of a stoichiometric approach such as flux balance analysis is that it can predict...

journal article 2009

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Opportunities for the integration of energy conservation in the asset management of landlords

Smid, J.W. (author), Nieboer, N. (author)

conference paper 2005

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Wonen in een kantoor: Deel 2

Smid, J.W. (author), de Jonge, T. (author)

journal article 2004

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Wonen in een kantoor: Deel 1

Smid, J.W. (author), de Jonge, T. (author)