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Velasco Alvarez, M.I. (author), ten Pierick, A. (author), van Dam, P.T.N. (author), Maleki Seifar, R. (author), van Loosdrecht, M.C.M. (author), Wahl, S.A. (author)
Poly(3-hydroxybutyrate) (PHB) is an interesting biopolymer for replacing petroleum-based plastics, its biological production is performed in natural and engineered microorganisms. Current metabolic engineering approaches rely on high-throughput strain construction and screening. Analytical procedures have to be compatible with the small scale...
journal article 2017
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Krishnan, S.T. (author), Moolman, M.C. (author), Van Laar, T. (author), Meyer, A.S. (author), Dekker, N.H. (author)
Background Chromosome engineering encompasses a collection of homologous recombination-based techniques that are employed to modify the genome of a model organism in a controlled fashion. Such techniques are widely used in both fundamental and industrial research to introduce multiple insertions in the same Escherichia coli strain. To date, ?...
journal article 2015