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Perli, T. (author), Moonen, Dewi P.I. (author), van den Broek, M.A. (author), Pronk, J.T. (author), Daran, J.G. (author)
Quantitative physiological studies on Saccharomyces cerevisiae commonly use synthetic media (SM) that contain a set of water-soluble growth factors that, based on their roles in human nutrition, are referred to as B vitamins. Previous work demonstrated that in S. cerevisiae CEN.PK113-7D, requirements for biotin were eliminated by laboratory...
journal article 2020
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Gorter de Vries, A.R. (author), Knibbe, E. (author), van Roosmalen, Roderick (author), van den Broek, M.A. (author), de la Torre, P. (author), O’Herne, Stephanie F. (author), Vijverberg, Pascal A. (author), el Masoudi, Anissa (author), Brouwers, N. (author), Pronk, J.T. (author), Daran, J.G. (author)
The lager-brewing yeast Saccharomyces pastorianus is a hybrid between S. cerevisiae and S. eubayanus with an exceptional degree of aneuploidy. While chromosome copy number variation (CCNV) is present in many industrial Saccharomyces strains and has been linked to various industrially-relevant traits, its impact on the brewing performance of S...
journal article 2020
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Valk, L.C. (author), Luttik, M.A.H. (author), de Ram, C. (author), Pabst, M. (author), van den Broek, M.A. (author), van Loosdrecht, Mark C.M. (author), Pronk, J.T. (author)
D-galacturonate, a key constituent of pectin, is a ubiquitous monomer in plant biomass. Anaerobic, fermentative conversion of D-galacturonate is therefore relevant in natural environments as well as in microbial processes for microbial conversion of pectin-containing agricultural residues. In currently known microorganisms that anaerobically...
journal article 2020
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Perli, T. (author), Wronska, A.K. (author), Ortiz Merino, R.A. (author), Pronk, J.T. (author), Daran, J.G. (author)
Chemically defined media for yeast cultivation (CDMY) were developed to support fast growth, experimental reproducibility, and quantitative analysis of growth rates and biomass yields. In addition to mineral salts and a carbon substrate, popular CDMYs contain seven to nine B-group vitamins, which are either enzyme cofactors or precursors for...
journal article 2020
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Feng, Cuijie (author), Welles, L. (author), Zhang, X. (author), Pronk, M. (author), de Graaff, D.R. (author), van Loosdrecht, Mark C.M. (author)
Phosphorus has been successfully eliminated from wastewater by biological techniques of enhanced biological phosphorus removal (EBPR) process, which relies on a specific microbiota of polyphosphate accumulating organisms (PAOs) that accumulate phosphate as polyphosphates (poly-P). Most methods for quantification of poly-P pools suffer from...
journal article 2020
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de Graaff, D.R. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)
Seawater can be introduced or intrude in sewer systems and can thereby negatively influence biological wastewater treatment processes. Here we studied the impact of artificial seawater on the enhanced biological phosphate removal (EBPR) process performance by aerobic granular sludge (AGS) with synthetic wastewater. Process performance,...
journal article 2020
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de Graaff, D.R. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)
Many sources of wastewater contain sulfides, which can cause excessive growth of filamentous bacteria such as Thiothrix sp. resulting in bulking sludge in conventional activated sludge systems. Granular sludge systems could potentially also suffer from the growth of filamentous bacteria. Uptake of easily degradable COD by the relatively slow...
journal article 2020
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Barrios Hernandez, M.L. (author), Pronk, M. (author), Garcia, H. (author), Boersma, Arne (author), Brdjanovic, Damir (author), van Loosdrecht, Mark C.M. (author), Hooijmans, Christine M. (author)
The aim of this study was to evaluate the effectiveness of the novel aerobic granular sludge (AGS) wastewater treatment technology in removing faecal indicator organisms (FIOs) compared to the conventional activated sludge (CAS) treatment system. The work was carried out at two full-scale wastewater treatment plants (WWTP) in the Netherlands,...
journal article 2020
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Wronska, A.K. (author), Haak, Meinske P. (author), Geraats, Ellen (author), Bruins Slot, Eva (author), van den Broek, M.A. (author), Pronk, J.T. (author), Daran, J.G. (author)
Biotin, an important cofactor for carboxylases, is essential for all kingdoms of life. Since native biotin synthesis does not always suffice for fast growth and product formation, microbial cultivation in research and industry often requires supplementation of biotin. De novo biotin biosynthesis in yeasts is not fully understood, which...
journal article 2020
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Valk, L.C. (author), Diender, Martijn (author), Stouten, G.R. (author), Petersen, Jette F. (author), Nielsen, Per H. (author), Dueholm, Morten S. (author), Pronk, J.T. (author), van Loosdrecht, Mark C.M. (author)
Acetogens have the ability to fixate carbon during fermentation by employing the Wood-Ljungdahl pathway (WLP), which is highly conserved across Bacteria and Archaea. In a previous study, product stoichometries in galacturonate-limited, anaerobic enrichment cultures of “Candidatus Galacturonibacter soehngenii,” from a novel genus within the...
journal article 2020
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Wiersma, S.J. (author), Mooiman, C. (author), Giera, Martin (author), Pronk, J.T. (author)
Biosynthesis of sterols, which are considered essential components of virtually all eukaryotic membranes, requires molecular oxygen. Anaerobic growth of the yeast Saccharomyces cerevisiae therefore strictly depends on sterol supplementation of synthetic growth media. Neocallimastigomycota are a group of strictly anaerobic fungi which, instead...
journal article 2020
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Haaksman, V.A. (author), Mirghorayshi, M. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)
Operational disturbances in aerobic granular sludge (AGS) systems can result in aerobic availability of readily biodegradable COD (rbCOD). Different from activated sludge, morphological consequences on the short and long term are not well described in literature. This study investigated the effect of incomplete anaerobic uptake of acetate on...
journal article 2020
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van Dijk, E.J.H. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author)
The settling behavior of aerobic granular sludge (AGS) in full-scale reactors is different from the settling of normal activated sludge. Current activated sludge models lack the features to describe the segregation of granules based on size during the settling process. This segregation plays an important role in the granulation process and...
journal article 2020
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van der Meij, Leander (author), Demetriou, A.M. (author), Tulin, Marina (author), Méndez, Ileana (author), Dekker, Peter (author), Pronk, Tila (author)
There is evidence that testosterone and cortisol levels are related to the attraction of a romantic partner; testosterone levels relate to a wide range of sexual behaviors and cortisol is a crucial component in the response to stress. To investigate this, we conducted a speed-dating study among heterosexual singles. We measured salivary...
journal article 2019
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Dekker, W.J.C. (author), Wiersma, S.J. (author), Bouwknegt, J. (author), Mooiman, C. (author), Pronk, J.T. (author)
In Saccharomyces cerevisiae, acyl-coenzyme A desaturation by Ole1 requires molecular oxygen. Tween 80, a poly-ethoxylated sorbitan-oleate ester, is therefore routinely included in anaerobic growth media as a source of unsaturated fatty acids (UFAs). During optimization of protocols for anaerobic bioreactor cultivation of this yeast, we...
journal article 2019
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Venturini, T. (author), Turrin, M. (author), Setaki, F. (author), Veer, F.A. (author), Pronk, Arno (author), Teuffel, Patrick (author), Moonen, Yaron (author), Slangen, Stefan (author), Vorstermans, Rens (author)
In recent years, natural disaster and military conflicts forced vast numbers of people to flee their home countries, contributing to the migration crisis we are facing today. According to the UNHCR, the number of forcibly displaced people worldwide reached the highest level since World War II. Post-disaster housing is by nature diverse and...
journal article 2019
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Brouwers, N. (author), Gorter de Vries, A.R. (author), van den Broek, M.A. (author), Weening, S.M. (author), Elink Schuurman, Tom D. (author), Kuijpers, Niels G.A. (author), Pronk, J.T. (author), Daran, J.G. (author)
Saccharomyces eubayanus is the non-S. cerevisiae parent of the lager-brewing hybrid S. pastorianus. In contrast to most S. cerevisiae and Frohberg-type S. pastorianus strains, S. eubayanus cannot utilize the α-tri-glucoside maltotriose, a major carbohydrate in brewer's wort. In Saccharomyces yeasts, utilization of maltotriose is encoded by...
journal article 2019
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Gorter de Vries, A.R. (author), Voskamp, Maaike (author), van Aalst, Aafke (author), Kristensen, L.H. (author), Jansen, Liset (author), Salazar, A.N. (author), van den Broek, M.A. (author), Brouwers, N. (author), Abeel, T.E.P.M.F. (author), Pronk, J.T. (author), Daran, J.G. (author)
Saccharomyces pastorianus lager-brewing yeasts are domesticated hybrids of S. cerevisiae x S. eubayanus that display extensive inter-strain chromosome copy number variation and chromosomal recombinations. It is unclear to what extent such genome rearrangements are intrinsic to the domestication of hybrid brewing yeasts and whether they...
journal article 2019
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Liu, Y. (author), El Masoudi, Anissa (author), Pronk, J.T. (author), van Gulik, W.M. (author)
So far, the physiology of Saccharomyces cerevisiae at near-zero growth rates has been studied in retentostat cultures with a growth-limiting supply of the carbon and energy source. Despite its relevance in nature and industry, the near-zero growth physiology of S. cerevisiae under conditions where growth is limited by the supply of non-energy...
journal article 2019
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Hakkaart, X.D.V. (author), Liu, Y. (author), Hulst, Mandy (author), el Masoudi, Anissa (author), Peuscher, Eveline (author), Pronk, J.T. (author), van Gulik, W.M. (author), Daran-Lapujade, P.A.S. (author)
Engineered strains of Saccharomyces cerevisiae are used for industrial production of succinic acid. Optimal process conditions for dicarboxylic-acid yield and recovery include slow growth, low pH, and high CO<sub>2</sub>. To quantify and understand how these process parameters affect yeast physiology, this study investigates individual and...
journal article 2019
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