"uuid","repository link","title","author","contributor","publication year","abstract","subject topic","language","publication type","publisher","isbn","issn","patent","patent status","bibliographic note","access restriction","embargo date","faculty","department","research group","programme","project","coordinates"
"uuid:27e20e06-0128-4201-aaf6-5f197b33f241","http://resolver.tudelft.nl/uuid:27e20e06-0128-4201-aaf6-5f197b33f241","Coupling Two Ultra-high-Speed Cameras to Elucidate Ultrasound Contrast-Mediated Imaging and Therapy","Li, H. (Erasmus MC); Li, X. (TU Delft ImPhys/Medical Imaging); Collado Lara, G. (Erasmus MC); Lattwein, K.R. (Erasmus MC); Mastik, Frits (Erasmus MC); Beurskens, Robert (Erasmus MC); van der Steen, A.F.W. (TU Delft ImPhys/Medical Imaging; Erasmus MC); Verweij, M.A. (TU Delft Technology, Policy and Management; Erasmus MC); de Jong, N. (TU Delft ImPhys/Medical Imaging; Erasmus MC); Kooiman, Klazina (Erasmus MC)","","2023","Ultrasound contrast-mediated medical imaging and therapy both rely on the dynamics of micron- and nanometer-sized ultrasound cavitation nuclei, such as phospholipid-coated microbubbles and phase-change droplets. Ultrasound cavitation nuclei respond non-linearly to ultrasound on a nanosecond time scale that necessitates the use of ultra-high-speed imaging to fully visualize these dynamics in detail. In this study, we developed an ultra-high-speed optical imaging system that can record up to 20 million frames per second (Mfps) by coupling two small-sized, commercially available, 10-Mfps cameras. The timing and reliability of the interleaved cameras needed to achieve 20 Mfps was validated using two synchronized light-emitting diode strobe lights. Once verified, ultrasound-activated microbubble responses were recorded and analyzed. A unique characteristic of this coupled system is its ability to be reconfigured to provide orthogonal observations at 10 Mfps. Acoustic droplet vaporization was imaged from two orthogonal views, by which the 3-D dynamics of the phase transition could be visualized. This optical imaging system provides the temporal resolution and experimental flexibility needed to further elucidate the dynamics of ultrasound cavitation nuclei to potentiate the clinical translation of ultrasound-mediated imaging and therapy developments.","Cavitation; Droplet; Microbubble; Ultra-high-speed imaging; Ultrasound; Ultrasound contrast agents","en","journal article","","","","","","","","","Technology, Policy and Management","","ImPhys/Medical Imaging","","",""
"uuid:ee49615f-4786-43c7-ad06-edd18463c0e1","http://resolver.tudelft.nl/uuid:ee49615f-4786-43c7-ad06-edd18463c0e1","Opening of endothelial cell–cell contacts due to sonoporation","Beekers, D.I. (Erasmus MC); Vegter, Merel (Erasmus MC); Lattwein, Kirby R. (Erasmus MC); Mastik, Frits (Erasmus MC); Beurskens, Robert (Erasmus MC); van der Steen, A.F.W. (TU Delft ImPhys/Medical Imaging; Erasmus MC); de Jong, N. (TU Delft ImPhys/Medical Imaging; Erasmus MC); Verweij, M.D. (TU Delft ImPhys/Medical Imaging; Erasmus MC); Kooiman, Klazina (Erasmus MC)","","2020","Ultrasound insonification of microbubbles can locally increase vascular permeability to enhance drug delivery. To control and optimize the therapeutic potential, we need to better understand the underlying biological mechanisms of the drug delivery pathways. The aim of this in vitro study was to elucidate the microbubble-endothelial cell interaction using the Brandaris 128 ultra-high-speed camera (up to 25 Mfps) coupled to a custom-built Nikon confocal microscope, to visualize both microbubble oscillation and the cellular response. Sonoporation and opening of cell-cell contacts by single αVβ3-targeted microbubbles (n = 152) was monitored up to 4 min after ultrasound insonification (2 MHz, 100–400 kPa, 10 cycles). Sonoporation occurred when microbubble excursion amplitudes exceeded 0.7 μm. Quantification of the influx of the fluorescent model drug propidium iodide upon sonoporation showed that the size of the created pore increased for larger microbubble excursion amplitudes. Microbubble-mediated opening of cell-cell contacts occurred as a cellular response upon sonoporation and did not correlate with the microbubble excursion amplitude itself. The initial integrity of the cell-cell contacts affected the susceptibly to drug delivery, since cell-cell contacts opened more often when cells were only partially attached to their neighbors (48%) than when fully attached (14%). The drug delivery outcomes were independent of nonlinear microbubble behavior, microbubble location, and cell size. In conclusion, by studying the microbubble–cell interaction at nanosecond and nanometer resolution the relationship between drug delivery pathways and their underlying mechanisms was further unraveled. These novel insights will aid the development of safe and efficient microbubble-mediated drug delivery.","Cell-cell contact opening; Drug delivery; High-speed imaging; Microbubbles; Sonoporation; Ultrasound","en","journal article","","","","","","","","","","","ImPhys/Medical Imaging","","",""
"uuid:1c5db92d-95c4-40e0-ba73-ab140f72c6cd","http://resolver.tudelft.nl/uuid:1c5db92d-95c4-40e0-ba73-ab140f72c6cd","High-Resolution Imaging of Intracellular Calcium Fluctuations Caused by Oscillating Microbubbles","Beekers, D.I. (Erasmus MC); Mastik, Frits (Erasmus MC); Beurskens, Robert (Erasmus MC); Tang, Phoei Ying (Erasmus MC); Vegter, Merel (Erasmus MC); van der Steen, A.F.W. (TU Delft ImPhys/Medical Imaging; Erasmus MC); de Jong, N. (TU Delft ImPhys/Medical Imaging; Erasmus MC); Verweij, M.D. (TU Delft ImPhys/Medical Imaging; Erasmus MC); Kooiman, Klazina (Erasmus MC)","","2020","Ultrasound insonification of microbubbles can locally enhance drug delivery, but the microbubble–cell interaction remains poorly understood. Because intracellular calcium (Cai 2+) is a key cellular regulator, unraveling the Cai 2+ fluctuations caused by an oscillating microbubble provides crucial insight into the underlying bio-effects. Therefore, we developed an optical imaging system at nanometer and nanosecond resolution that can resolve Cai 2+ fluctuations and microbubble oscillations. Using this system, we clearly distinguished three Cai 2+ uptake profiles upon sonoporation of endothelial cells, which strongly correlated with the microbubble oscillation amplitude, severity of sonoporation and opening of cell–cell contacts. We found a narrow operating range for viable drug delivery without lethal cell damage. Moreover, adjacent cells were affected by a calcium wave propagating at 15 μm/s. With the unique optical system, we unraveled the microbubble oscillation behavior required for drug delivery and Cai 2+ fluctuations, providing new insight into the microbubble–cell interaction to aid clinical translation.","Cell–cell contact opening; Confocal microscopy; Drug delivery; High-speed imaging; Intracellular calcium; Microbubbles; Sonoporation; Ultrasound","en","journal article","","","","","","","","","","","ImPhys/Medical Imaging","","",""
"uuid:361f73a0-9c25-4e68-9671-9eed129df834","http://resolver.tudelft.nl/uuid:361f73a0-9c25-4e68-9671-9eed129df834","Combined Confocal Microscope and Brandaris 128 Ultra-High-Speed Camera","Beekers, D.I. (Erasmus MC); Lattwein, Kirby R. (Erasmus MC); Kouijzer, Joop J.P. (Erasmus MC); Langeveld, Simone A.G. (Erasmus MC); Vegter, M. (Erasmus MC); Beurskens, Robert (Erasmus MC); Mastik, F. (Erasmus MC); Verduyn Lunel, Rogier (Nikon, Amsterdam); van der Steen, A.F.W. (TU Delft ImPhys/Acoustical Wavefield Imaging; Erasmus MC); de Jong, N. (TU Delft ImPhys/Acoustical Wavefield Imaging; Erasmus MC)","","2019","Controlling microbubble-mediated drug delivery requires the underlying biological and physical mechanisms to be unraveled. To image both microbubble oscillation upon ultrasound insonification and the resulting cellular response, we developed an optical imaging system that can achieve the necessary nanosecond temporal and nanometer spatial resolutions. We coupled the Brandaris 128 ultra-high-speed camera (up to 25 million frames per second) to a custom-built Nikon A1R+ confocal microscope. The unique capabilities of this combined system are demonstrated with three experiments showing microbubble oscillation leading to either endothelial drug delivery, bacterial biofilm disruption, or structural changes in the microbubble coating. In conclusion, using this state-of-the-art optical imaging system, microbubble-mediated drug delivery can be studied with high temporal resolution to resolve microbubble oscillation and high spatial resolution and detector sensitivity to discern cellular response. Combining these two imaging technologies will substantially advance our knowledge on microbubble behavior and its role in drug delivery.","Bacteria; Confocal microscopy; Drug delivery; Fluorescence microscopy; High-speed imaging; Lipid coating; Microbubble; Sonoporation; Ultrasound; Ultrasound contrast agents","en","journal article","","","","","","","","","","","ImPhys/Acoustical Wavefield Imaging","","",""
"uuid:47632dfc-4c6a-4860-8a7b-d803d5bc6395","http://resolver.tudelft.nl/uuid:47632dfc-4c6a-4860-8a7b-d803d5bc6395","Combined optical sizing and acoustical characterization of single freely-floating microbubbles","Luan, Ying (Erasmus MC); Renaud, Guillaume (Erasmus MC; UPMC-Sorbonne Universités & CNRS); Raymond, Jason L. (Erasmus MC); Segers, Tim (University of Twente); Lajoinie, Guillaume (University of Twente); Beurskens, Robert (Erasmus MC); Mastik, Frits (Erasmus MC); Kokhuis, Tom J A (Erasmus MC); van der Steen, A.F.W. (TU Delft ImPhys/Acoustical Wavefield Imaging; Erasmus MC); Versluis, Michel (University of Twente); de Jong, N. (TU Delft ImPhys/Acoustical Wavefield Imaging; Erasmus MC)","","2016","In this study we present a combined optical sizing and acoustical characterization technique for the study of the dynamics of single freely-floating ultrasound contrast agent microbubbles exposed to long burst ultrasound excitations up to the milliseconds range. A co-axial flow device was used to position individual microbubbles on a streamline within the confocal region of three ultrasound transducers and a high-resolution microscope objective. Bright-field images of microbubbles passing through the confocal region were captured using a high-speed camera synchronized to the acoustical data acquisition to assess the microbubble response to a 1-MHz ultrasound burst. Nonlinear bubble vibrations were identified at a driving pressure as low as 50 kPa. The results demonstrate good agreement with numerical simulations based on the shell-buckling model proposed by Marmottant et al. [J. Acoust. Soc. Am. 118, 3499-3505 (2005)]. The system demonstrates the potential for a high-throughput in vitro characterization of individual microbubbles.","Ultranonography; Bubble dynamics; Nonlinear dynamics; Vibration testing; Cameras","en","journal article","","","","","","","","2017-12-07","","","ImPhys/Acoustical Wavefield Imaging","","",""
"uuid:c21d8633-397b-4ba8-95d4-bcbb4fa79f80","http://resolver.tudelft.nl/uuid:c21d8633-397b-4ba8-95d4-bcbb4fa79f80","High-definition imaging of carotid artery wall dynamics","Kruizinga, P. (Erasmus MC); Mastik, Frits (Erasmus MC); van den Oord, Stijn C.H. (Erasmus MC); Schinkel, Arend F.L. (Erasmus MC); Bosch, Johannes G. (Erasmus MC); de Jong, N. (TU Delft ImPhys/Acoustical Wavefield Imaging; Erasmus MC; Interuniversity Cardiology Institute of the Netherlands); van Soest, G. (Erasmus MC); van der Steen, A.F.W. (TU Delft ImPhys/Acoustical Wavefield Imaging; Erasmus MC; Chinese Academy of Sciences; Interuniversity Cardiology Institute of the Netherlands)","","2014","The carotid artery (CA) is central to cardiovascular research, because of the clinical relevance of CA plaques as culprits of stroke and the accessibility of the CA for cardiovascular screening. The viscoelastic state of this artery, essential for clinical evaluation, can be assessed by observing arterial deformation in response to the pressure changes throughout the cardiac cycle. Ultrasound imaging has proven to be an excellent tool to monitor these dynamic deformation processes. We describe how a new technique called high-frame-rate ultrasound imaging captures the tissue deformation dynamics throughout the cardiac cycle in unprecedented detail. Local tissue motion exhibits distinct features of sub-micrometer displacements on a sub-millisecond time scale. We present a high-definition motion analysis technique based on plane wave ultrasound imaging able to capture these features. We validated this method by screening a group of healthy volunteers and compared the results with those for two patients known to have atherosclerosis to illustrate the potential utility of this technique.","Carotid artery; High-frame-rate ultrasound; Plane wave imaging; Pulse wave velocity; Tissue Doppler","en","journal article","","","","","","","","2015-10-01","","","ImPhys/Acoustical Wavefield Imaging","","",""