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Gisler, Santiago (author), P. Gonçalves, Joana (author), Akhtar, Waseem (author), de Jong, Johann (author), Pindyurin, Alexey V. (author), Wessels, L.F.A. (author), van Lohuizen, Maarten (author)Understanding the impact of guide RNA (gRNA) and genomic locus on CRISPR-Cas9 activity is crucial to design effective gene editing assays. However, it is challenging to profile Cas9 activity in the endogenous cellular environment. Here we leverage our TRIP technology to integrate ~ 1k barcoded reporter genes in the genomes of mouse embryonic...journal article 2019
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Komor, Malgorzata A. (author), Bosch, Linda J.W. (author), Bounova, Gergana (author), Bolijn, Anne S. (author), Delis‐van Diemen, Pien M. (author), Rausch, Christian (author), Hoogstrate, Youri (author), Stubbs, Andrew P. (author), de Jong, Mark (author), Wessels, L.F.A. (author)Consensus molecular subtyping is an RNA expression-based classification system for colorectal cancer (CRC). Genomic alterations accumulate during CRC pathogenesis, including the premalignant adenoma stage, leading to changes in RNA expression. Only a minority of adenomas progress to malignancies, a transition that is associated with specific...journal article 2018
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Cioni, Bianca (author), Nevedomskaya, Ekaterina (author), Melis, Monique H.M. (author), van Burgsteden, Johan (author), Stelloo, Suzan (author), Hodel, Emma (author), Spinozzi, Daniele (author), de Jong, Jeroen (author), Wessels, L.F.A. (author)Fibroblasts are abundantly present in the prostate tumor microenvironment (TME), including cancer-associated fibroblasts (CAFs) which play a key role in cancer development. Androgen receptor (AR) signaling is the main driver of prostate cancer (PCa) progression, and stromal cells in the TME also express AR. High-grade tumor and poor clinical...journal article 2018