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Giraud, G. (author), Schulze, H. (author), Li, D.U. (author), Bachmann, T.T. (author), Crain, J. (author), Tyndall, D. (author), Richardson, J. (author), Walker, R. (author), Stoppa, D. (author), Charbon, E. (author), Henderson, R. (author), Arlt, J. (author)
Fluorescence lifetime of dye molecules is a sensitive reporter on local microenvironment which is generally independent of fluorophores concentration and can be used as a means of discrimination between molecules with spectrally overlapping emission. It is therefore a potentially powerful multiplexed detection modality in biosensing but requires...
journal article 2010
document
Li, D.U. (author), Arlt, J. (author), Richardson, J. (author), Walker, R. (author), Buts, A. (author), Stoppa, D. (author), Charbon, E. (author), Henderson, R. (author)
A compact real-time fluorescence lifetime imaging microscopy (FLIM) system based on an array of low dark count 0.13?m CMOS singlephoton avalanche diodes (SPADs) is demonstrated. Fast background-insensitive fluorescence lifetime determination is achieved by use of a recently proposed algorithm called ‘Integration for Extraction Method’ (IEM) [J....
journal article 2010