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Zhou, Pengfei (author), Wang, Xuping (author), Yang, Bo (author), Hollmann, F. (author), Wang, Yonghua (author)Epoxides are important synthetic intermediates for the synthesis of a broad range of industrial products. This study presents a promising solution to the current limitation of enzyme instability. By using simple deep eutectic solvents such as choline chloride/sorbitol, significant stabilization of the biocatalyst has been achieved leading to...journal article 2017
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Lan, Dongming (author), Wang, Xuping (author), Zhou, Pengfei (author), Hollmann, F. (author), Wang, Yonghua (author)Deep eutectic solvents act as surfactants in biphasic (hydrophobic/aqueous) reaction mixtures enabling higher interfacial surface areas at lower mechanical stress as compared to simple emulsions. Exploiting this effect the rate of a chemoenzymatic epoxidation reaction was increased more than six-fold.journal article 2017
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Wang, Xu Ping (author), Zhou, Peng Fei (author), Li, Zhi Gang (author), Yang, Bo (author), Hollmann, F. (author), Wang, Yong Hua (author)A Ser105Ala mutant of the lipase B from Candida antarctica enables 'perhydrolase-only' reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially eliminating the undesired hydrolysis reaction of...journal article 2017
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Zhou, Pengfei (author), Wang, Xuping (author), Zeng, Chaoxi (author), Wang, Weifei (author), Yang, Bo (author), Hollmann, F. (author), Wang, Yonghua (author)A chemoenzymatic method for the production of epoxidized vegetable oils was developed. The unique combination of the commercial lipase G from Penicillieum camembertii with certain deep eutectic solvents enabled the efficient production of epoxidized vegetable oils.journal article 2017
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Wang, Yonghua (author), Lan, Dongming (author), Durrani, Rabia (author), Hollmann, F. (author)Peroxygenases are promising catalysts for preparative oxyfunctionalization chemistry as they combine the versatility of P450 monooxygenases with simplicity of cofactor-independent enzymes. Though many interesting applications have been reported, today ‘we have only scratched the surface’ and significant efforts are necessary to solve issues...review 2017
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Liu, Nan (author), Liu, D. (author), Wang, Weifei (author), Hollmann, F. (author), Xu, Long (author), Ma, Yunjian (author), Yang, Bo (author), Bai, Weidong (author), Sun, Xiaotao (author), Wang, Yonghua (author)α‐Linolenic acid‐rich diacylglycerol has been demonstrated with promising health promotion functions. This study examined the production, immobilization of lipase PCL, and its application in the synthesis of diacylglycerol by esterification of α‐linolenic acid with glycerol. The resin ECR8806 was selected as an effective support for the...journal article 2018
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Dong, J. (author), Fernandez Fueyo, E. (author), Hollmann, F. (author), Paul, C.E. (author), Pesic, M. (author), Schmidt, S. (author), Wang, Yonghua (author), Younes, S.H.H. (author), Zhang, W. (author)Oxidation chemistry using enzymes is approaching maturity and practical applicability in organic synthesis. Oxidoreductases (enzymes catalysing redox reactions) enable chemists to perform highly selective and efficient transformations ranging from simple alcohol oxidations to stereoselective halogenations of non‐activated C−H bonds. For many of...journal article 2018
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Hollmann, F. (author), Kara, Selin (author), Opperman, Diederik J. (author), Wang, Yonghua (author)Cyclic esters and amides (lactones and lactams) are important active ingredients and polymer building blocks. In recent years, numerous biocatalytic methods for their preparation have been developed including enzymatic and chemoenzymatic Baeyer–Villiger oxidations, oxidative lactonisation of diols, and reductive lactonisation and...journal article 2018
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Younes, S.H.H. (author), Tieves, F. (author), Lan, Dongming (author), Wang, Yonghua (author), Süss, Philipp (author), Brundiek, Henrike (author), Wever, Ron (author), Hollmann, F. (author)A chemoenzymatic method for the halocyclization of unsaturated alcohols and acids by using the robust V-dependent chloroperoxidase from Curvularia inaequalis (CiVCPO) as catalyst has been developed for the in situ generation of hypohalites. A broad range of halolactones and cyclic haloethers are formed with excellent performance of the...journal article 2019
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Zhang, Tianyu (author), Ma, Yunjian (author), Tan, Chin Ping (author), Hollmann, F. (author), Wang, Jianrong (author), Yang, Bo (author), Wang, Yonghua (author)Poor H <sub>2</sub> O <sub>2</sub> -resistance by enzymes is a key bottleneck in the epoxidation process of oil by enzymatic methods. In this study, the stability of three lipases, from Aspergillus oryzae lipase (AOL), Aspergillus fumigatus lipase B (AflB), and marine Janibacter (MAJ1), in the presence of H <sub>2</sub> O <sub>2</sub> was...journal article 2019
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Zhao, Ze Xin (author), Lan, Dongming (author), Tan, Xiyu (author), Hollmann, F. (author), Bornscheuer, Uwe T. (author), Yang, Bo (author), Wang, Yonghua (author)H <sub>2</sub> O <sub>2</sub> , is an attractive oxidant for synthetic chemistry, especially if activated as percarboxylic acid. H <sub>2</sub> O <sub>2</sub> , however, is also a potent inactivator of enzymes. Protein engineering efforts to improve enzyme resistance against H <sub>2</sub> O <sub>2</sub> in the past have mostly focused on...journal article 2019
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Li, Yongru (author), Ma, Yunjian (author), Li, Peilin (author), Zhang, Xizhen (author), Ribitsch, Doris (author), Alcalde, Miguel (author), Hollmann, F. (author), Wang, Yonghua (author)A bienzymatic cascade for selective sulfoxidation is presented. The evolved recombinant peroxygenase from Agrocybe aegeritra catalyses the enantioselective sulfoxidation of thioanisole whereas the choline oxidase from Arthrobacter nicotianae provides the H2 O2 necessary via reductive activation of ambient oxygen. The reactions are performed...journal article 2020
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Ma, Yunjian (author), Zhang, Xizhen (author), Li, Yongru (author), Li, Peilin (author), Hollmann, F. (author), Wang, Yonghua (author)A biocatalytic cascade transforming castor oil into (R,Z)-octadec-9-en-7-ol is presented by combining a lipase catalysed hydrolysis of castor oil into ricinoleic acid followed by a photoenzymatic decarboxylation into (R,Z)-octadec-9-en-7-ol. Conversion of up to 41.7% and overall product concentrations of up to 60 mM, this new bienzymatic and...journal article 2020
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Ma, Yunjian (author), Zhang, Xizhen (author), Zhang, W. (author), Li, Peilin (author), Li, Yongru (author), Hollmann, F. (author), Wang, Yonghua (author)A photobiocatalytic cascade transforming natural triglycerides into alkanes/alkenes is proposed. Starting from natural triglycerides, free fatty acids have been obtained using lipases. The free fatty acids were then, in a photoenzymatic step, decarboxylated into the C1-shortened alkanes using a recently described photodecarboxylase from...journal article 2020
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Chen, Shaohang (author), Zhang, Jiaan (author), Zeng, Zhigang (author), Dai, Zongjie (author), Wang, Qinhong (author), Wever, Ron (author), Hollmann, F. (author), Zhang, W. (author)A chemoenzymatic method for the synthesis of haloethers is presented. A combination of enzymatic hypohalite synthesis with spontaneous oxidation of alkenes and nucleophilic attack by various alcohols enabled the synthesis of a wide range of haloethers. The reaction system has been characterised and current imitations have been worked out. In...journal article 2022
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Ma, Yunjian (author), Liang, Hongjing (author), Zhao, Zexin (author), Wu, Bin (author), Lan, Dongming (author), Hollmann, F. (author), Wang, Yonghua (author)Unspecific peroxygenases (UPOs, EC 1.11.2.1) are promising oxyfunctionalization catalysts because of their unique stereoselectivity. However, so far only a few UPOs have been reported. In this study, gene mining was used to identify a gene from Galerina marginata that coded for a novel UPO (GmaUPO). GmaUPO was expressed in Pichia pastoris X...journal article 2022
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Zhong, Xuanru (author), Ma, Yunjian (author), Zhang, Xizhen (author), Zhang, Jiahui (author), Wu, Bin (author), Hollmann, F. (author), Wang, Yonghua (author)Hydrocarbon synthesis from (waste)oils enabled by a cascade of lipase-catalysed hydrolysis and decarboxylase-catalysed decarboxylation has become an active area of research en route to alternative, biobased fuels. However, Poor substrate transport efficiency is a major issue causing low reaction rates. This study focused on a protein self...journal article 2022
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Li, Zongquan (author), Ma, Yunjian (author), Hollmann, F. (author), Wang, Yonghua (author)Carvol and carvone are oxidation products from the natural product limonene. They are important raw materials for the flavours and fragrances industry and also act as pharmaceutical active ingredients. Orange waste peels possibly represent an attractive source for limonene, but studies on valorizing orange peel wastes are rare. In this study,...journal article 2022
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Yang, Liu (author), Ma, Yunjian (author), Chen, Yebao (author), Hollmann, F. (author), Wang, Yonghua (author)Phthalic acid esters (PAEs) are widely used as plastic additives to increase the flexibility and durability of plastics. Constantly leaching out from plastics, PAEs are ubiquitously found in the environment. As PAEs exhibit biological activities such as being endocrine disruptive, the quest for efficient degradation strategies continues. Here...journal article 2022
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Wu, Bin (author), Wang, S. (author), Ma, Yunjian (author), Yuan, Shuguang (author), Hollmann, F. (author), Wang, Yonghua (author)Alcohol oxidases (AOxs) catalyze the aerobic oxidation of alcohols to the corresponding carbonyl products (aldehydes or ketones), producing only H<sub>2</sub>O<sub>2</sub> as the byproduct. The majority of known AOxs, however, have a strong preference for small, primary alcohols, limiting their broad applicability, e.g., in the food industry....journal article 2023
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