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Fanalista, F. (author), Birnie, A.T.F. (author), Maan, R. (author), Burla, Federica (author), Charles usagé Charles-Carty, K.V. (author), Pawlik, G. (author), Deshpande, S.R. (author), Koenderink, Gijsje H. (author), Dogterom, A.M. (author), Dekker, C. (author)
Bottom-up biology is an expanding research field that aims to understand the mechanisms underlying biological processes via in vitro assembly of their essential components in synthetic cells. As encapsulation and controlled manipulation of these elements is a crucial step in the recreation of such cell-like objects, microfluidics is...
journal article 2019
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Schaich, M. (author), Cama, J. (author), Al Nahas, K. (author), Sobota, Diana (author), Sleath, Hannah (author), Jahnke, Kevin (author), Deshpande, S.R. (author), Dekker, C. (author), Keyser, Ulrich F. (author)
The low membrane permeability of candidate drug molecules is a major challenge in drug development, and insufficient permeability is one reason for the failure of antibiotic treatment against bacteria. Quantifying drug transport across specific pathways in living systems is challenging because one typically lacks knowledge of the exact...
journal article 2019
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Deshpande, S.R. (author), Wunnava Venkata, S.S. (author), Hueting, D.A. (author), Dekker, C. (author)
Recent years have seen a tremendous interest in the bottom-up reconstitution of minimal biomolecular systems, with the ultimate aim of creating an autonomous synthetic cell. One of the universal features of living systems is cell growth, where the cell membrane expands through the incorporation of newly synthesized lipid molecules. Here, the...
journal article 2019
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Liu, Q. (author), Zhao, M. (author), Mytnyk, S. (author), Klemm, B. (author), Zhang, K. (author), Wang, Y. (author), Yan, Dadong (author), Mendes, E. (author), van Esch, J.H. (author)
Hydrogel microparticles are important in materials engineering, but their applications remain limited owing to the difficulties associated with their manipulation. Herein, we report the self-orientation of crescent-shaped hydrogel microparticles and elucidate its mechanism. Additionally, the microparticles were used, for the first time, as...
journal article 2019
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Bui, T.H. (author)
The thesis focuses on the investigation of thin-film surface acoustic wave (SAW) devices for liquid sensing applications. The piezoelectric material is a thin film of Aluminum Nitride (AlN), a CMOS compatible material, deposited by pulse DC reactive sputtering technique. A CMOS compatible process is developed and employed to fabricate the AlN/Si...
doctoral thesis 2018
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Marin Lizarraga, V.M. (author)
This thesis presents a reliable technology to assemble free standing lipid membranes using microfabricated devices. A microfluidic cartridge consisting of parallel channels connected with a rectangular aperture was designed and characterized to assemble artificial membranes. This methodology resulted on a system capable of assembling lipid...
doctoral thesis 2018
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Deshpande, S.R. (author), Spoelstra, W.K. (author), Kerssemakers, J.W.J. (author), Dekker, C. (author)
Liposomes, self-assembled vesicles with a lipid-bilayer boundary similar to cell membranes, are extensively used in both fundamental and applied sciences. Manipulation of their physical properties, such as growth and division, may significantly expand their use as model systems in cellular and synthetic biology. Several approaches have been...
journal article 2018
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Kawale, D. (author)
Crude oil has been an important source of energy for several decades now. Upon discovery of a crude oil reservoir until its abandonment, typically the oil recovery process can be classified in three stages. The primary and the secondary stage involve utilizing and maintaining reservoir pressure to recover oil respectively. The tertiary, or the...
doctoral thesis 2017
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Tiruvadi Krishnan, S. (author)
All living organisms pass on their genetic information to their offspring in the form of DNA or RNA molecules by duplicating them across generations. In the bacteria, their genes are packed in long chains of DNA molecules or chromosomes. One of the widely studied model organisms, Escherichia coli, replicates its circular chromosome in two...
doctoral thesis 2016
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De Martino, M. (author)
doctoral thesis 2016
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Gude, S. (author)
Quantitative experiments with the ability to systematically probe the system under investigation are a key element to gain insight and understanding. We present a novel and simple technique enabling the maintenance and controlled perturbation of experimental conditions of microorganisms from bacteria to nematodes. Polyacrylamide-based devices...
doctoral thesis 2016
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Wu, F. (author)
One of the most basic features that pervade biology is the existence of boundaries that separate living cells from their outer environments. Molecules responsible for the internal organization of a living cell must adapt to its boundaries, as the cell grows, divides, and changes in shape and size. In this thesis, we aim to understand how...
doctoral thesis 2015
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Pérez Garza, H. (author), Ghatkesar, M.K. (author), Basak, S. (author), Löthman, P. (author), Staufer, U. (author)
To manipulate liquid matter at the nanometer scale, we have developed a robotic assembly equipped with a hollow atomic force microscope (AFM) cantilever that can handle femtolitre volumes of liquid. The assembly consists of four independent robots, each sugar cube sized with four degrees of freedom. All robots are placed on a single platform...
journal article 2015
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Ghatkesar, M.K. (author), Perez Garza, H.H. (author), Heuck, F. (author), Staufer, U. (author)
Advances in micro and nano fabrication technologies have enabled fabrication of smaller and more sensitive devices for applications not only in solid-state physics but also in medicine and biology. The demand for devices that can precisely transport material, specifically fluids are continuously increasing. Therefore, integration of various...
journal article 2014
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Van Vliet, S. (author), Hol, F.J.H. (author), Weenink, T. (author), Galajda, P. (author), Keymer, J.E. (author)
Background Bacterial habitats, such as soil and the gut, are structured at the micrometer scale. Important aspects of microbial life in such spatial ecosystems are migration and colonization. Here we explore the colonization of a structured ecosystem by two neutrally labeled strains of Escherichia coli. Using time-lapse microscopy we studied the...
journal article 2014
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Chen, P.W. (author), Neuser, S. (author), Michaud, V. (author), Studart, A.R. (author)
Abstract only.
conference paper 2013
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Boulineau, S. (author)
doctoral thesis 2013
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Moolman, M.C. (author), Huang, Z. (author), Krishnan, S.T. (author), Kerssemakers, J.W.J. (author), Dekker, N.H. (author)
Background: Controlled restriction of cellular movement using microfluidics allows one to study individual cells to gain insight into aspects of their physiology and behaviour. For example, the use of micron-sized growth channels that confine individual Escherichia coli has yielded novel insights into cell growth and death. To extend this...
journal article 2013
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Hoang, D.A. (author), Portela, L.M. (author), Kleijn, C.R. (author), Kreutzer, M.T. (author), Van Steijn, V. (author)
The breakup of droplets due to creeping motion in a confined microchannel geometry is studied using three-dimensional numerical simulations. Analogously to unconfined droplets, there exist two distinct breakup phases: (i) a quasi-steady droplet deformation driven by the externally applied flow; and (ii) a surface-tension-driven three-dimensional...
journal article 2013
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Huang, Z. (author)
Heterogeneity is a general feature in biological system. In order to avoid possible misleading effects of ensemble averaging, and to ensure a correct understanding of the biological system, it is very important to look into individuals, such as a single bio-molecule or a single cell, for details. The size of a single bio-molecule/cell typically...
doctoral thesis 2012
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