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Allaart, M.T. (author), Korkontzelos, Charilaos (author), Sousa, Diana Z. (author), Kleerebezem, R. (author)
Syngas fermentation has gained momentum over the last decades. The cost-efficient design of industrial-scale bioprocesses is highly dependent on quantitative microbial growth data. Kinetic and stoichiometric models for syngas-converting microbes exist, but accurate experimental validation of the derived parameters is lacking. Here, we...
journal article 2024
document
Totlani, K. (author), van Tatenhove-Pel, R.J. (author), Kreutzer, M.T. (author), van Gulik, W.M. (author), van Steijn, V. (author)
It is common practice in the development of bioprocesses to genetically modify a microorganism and study a large number of resulting mutants in order to select the ones that perform best for use at the industrial scale. At industrial scale, strict nutrient-controlled growth conditions are imposed to control the metabolic activity and growth...
review 2023
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Minden, Steven (author), Aniolek, Maria (author), Sarkizi Shams Hajian, Christopher (author), Teleki, Attila (author), Zerrer, Tobias (author), Delvigne, Frank (author), van Gulik, W.M. (author), Deshmukh, Amit (author), Noorman, H.J. (author), Takors, Ralf (author)
Carbon limitation is a common feeding strategy in bioprocesses to enable an efficient microbiological conversion of a substrate to a product. However, industrial settings inherently promote mixing insufficiencies, creating zones of famine conditions. Cells frequently traveling through such regions repeatedly experience substrate shortages and...
journal article 2022
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Rombouts, J.L. (author)
Microbial fermentations are a key process in naturally and man-made ecosystems. Microbial fermentations play a key role in creating and digesting our food and they are useful in designing bioprocesses that can produce biogas, biofuels, bioplastics, and many other functional molecules (Chapter 1). Furthermore, studying the competition and...
doctoral thesis 2020
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Rombouts, J.L. (author), Mos, Galvin (author), Weissbrodt, D.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)
Efficient industrial fermentation of lignocellulosic waste containing a large part of glucose and xylose is desirable to implement a circular economy. Mixed culture biotechnologies can aid in realizing this goal. The effect of feeding equivalent substrates to a microbial community, such a xylose and glucose, is not well understood in terms of...
journal article 2019
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van den Berg, E.M. (author)
The anthropogenic nitrogen inputs in the environment exceed the input by natural processes and impact the global nitrogen cycle considerably . Human meddling in the N-cycle occurs mainly in agricultural ecosystems. Loss of nitrogen from the agricultural soils, other than crop harvest, can have polluting effects on other environments. The three...
doctoral thesis 2017
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van den Berg, E.M. (author), Perdigão Elisiário, M. (author), Kuenen, J.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)
Denitrification and dissimilatory reduction to ammonium (DNRA) are competing nitrate-reduction processes that entail important biogeochemical consequences for nitrogen retention/removal in natural and man-made ecosystems. The nature of the available carbon source and electron donor have been suggested to play an important role on the outcome of...
journal article 2017
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van den Berg, E.M. (author), Rombouts, J.L. (author), Kuenen, J.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)
Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) are two microbial processes that compete for oxidized nitrogen compounds in the environment. The objective of this work was to determine the role of nitrite versus nitrate as terminal electron acceptor on the competition between DNRA and denitrification. Initially, a mixed...
journal article 2017
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van den Berg, E.M. (author), Boleij, M. (author), Kuenen, J.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)
Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) compete for nitrate in natural and engineered environments. A known important factor in this microbial competition is the ratio of available electron donor and elector acceptor, here expressed as Ac/N ratio (acetate/nitrate-nitrogen). We studied the impact of the Ac/N ratio...
journal article 2016
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Lameiras, F. (author), Heijnen, J.J. (author), Van Gulik, W.M. (author)
In view of the high citric acid production capacity of Aspergillus niger, it should be well suited as a cell factory for the production of other relevant acids as succinic, fumaric, itaconic and malic. Quantitative metabolomics is an important omics tool in a synthetic biology approach to develop A. niger for the production of these acids. Such...
journal article 2015
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Hanke, A. (author), Hamann, E. (author), Dharma, R. (author), Geelhoed, J.S. (author), Hargesheimer, T. (author), Kraft, B. (author), Meyer, V. (author), Lenk, S. (author), Osmers, H. (author), Wu, R. (author), Makinwa, K.A.A. (author), Hettich, R.L. (author), Banfield, J.F. (author), Tegetmeyer, H.E. (author), Strous, M. (author)
Sandy coastal sediments are global hotspots for microbial mineralization of organic matter and denitrification. These sediments are characterized by advective porewater flow, tidal cycling and an active and complex microbial community. Metagenomic sequencing of microbial communities sampled from such sediments showed that potential sulfur...
journal article 2014
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Carnicer,, M. (author), Canelas, A.B. (author), Ten Pierick, A. (author), Zeng, Z. (author), Van Dam, J. (author), Albiol, J. (author), Ferrer, P. (author), Heijnen, J.J. (author), Van Gulik, W. (author)
Accurate, reliable and reproducible measurement of intracellular metabolite levels has become important for metabolic studies of microbial cell factories. A first critical step for metabolomic studies is the establishment of an adequate quenching and washing protocol, which ensures effective arrest of all metabolic activity and removal of...
journal article 2011
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Harris, D.M. (author)
With an over 1000-fold improvement in specific productivity since its discovery, penicillin is one of the very successful examples of industrial biotechnology. Although classical strain improvement programmes have been a major contributor to this success, the wish for a more rational approach towards improvements has driven the work described in...
doctoral thesis 2008
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Tai, S.L. (author)
This thesis is a compilation of a four-year PhD project on bakers' yeast (Saccharomyces cerevisiae). Since the entire S. cerevisiae genome sequence became available in 1996, DNA-microarray analysis has become a popular high-information-density tool for analyzing gene expression in this important industrial microorganism and model eukaryote. This...
doctoral thesis 2007
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Hensing, M.C.M. (author), Vrouwenvelder, J.S. (author), Hellinga, C. (author), van Dijken, J.P. (author), Pronk, J.T. (author)
journal article 1995
document
Van Niel, E.W.J. (author), Braber, K.J. (author), Robertson, L.A. (author), Kuenen, J.G. (author)
journal article 1992
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