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Daalman, W.K. (author), Sweep, E. (author), Laan, L. (author)Accurate phenotype prediction based on genetic information has numerous societal applications, such as crop design or cellular factories. Epistasis, when biological components interact, complicates modelling phenotypes from genotypes. Here we show an approach to mitigate this complication for polarity establishment in budding yeast, where...journal article 2023
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Liu, H. (author), Nadar, R.A. (author), Fauzia, R.P. (author), Laan, A.C. (author), Wang, R. (author), van Cooten, Quenteijn (author), Carroll, E.C.M. (author), Eelkema, R. (author), Denkova, A.G. (author)Combination of therapies is a common strategy in cancer treatment. Such combined therapies only have merit provided that there is superior therapeutic outcome with fewer side effects, compared to single therapies. Here, this work explores the possibility to combine chemotherapy with radionuclide therapy using polymeric micelles as a delivery...journal article 2023
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Hart-Davis, Michael G. (author), Laan, Stendert (author), Schwatke, Christian (author), Backeberg, Björn (author), Dettmering, Denise (author), Zijl, Firmijn (author), Verlaan, M. (author), Passaro, Marcello (author), Seitz, Florian (author)With the continued rise in global mean sea level, operational predictions of tidal height and total water levels have become crucial for accurate estimations and understanding of sea level processes. The Dutch Continental Shelf Model in Delft3D Flexible Mesh (DCSM-FM) is developed at Deltares to operationally estimate the total water levels...journal article 2023
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Kingma, E. (author), Diepeveen, E.T. (author), Iñigo de la Cruz, L.M. (author), Laan, L. (author)The ability of cells to translate different extracellular cues into different intracellular responses is vital for their survival in unpredictable environments. In Saccharomyces cerevisiae, cell polarity is modulated in response to environmental signals which allows cells to adopt varying morphologies in different external conditions. The...journal article 2023
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Biemolt, J. (author), Meeus, Eva J. (author), de Zwart, Felix J. (author), de Graaf, Jeen (author), Laan, Petrus C.M. (author), de Bruin, Bas (author), Burdyny, T.E. (author), Rothenberg, Gadi (author), Yan, Ning (author)Immobilizing molecular catalysts on electrodes is vital for electrochemical applications. However, creating robust electrode-catalyst interactions while maintaining good catalytic performance and rapid electron transfer is challenging. Here, without introducing any foreign elements, we show a bottom-up synthetic approach of constructing the...journal article 2023
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Brauns, Fridtjof (author), Iñigo de la Cruz, L.M. (author), Daalman, W.K. (author), de Bruin, Ilse (author), Halatek, Jacob (author), Laan, L. (author), Frey, Erwin (author)How can a self-organized cellular function evolve, adapt to perturbations, and acquire new sub-functions? To make progress in answering these basic questions of evolutionary cell biology, we analyze, as a concrete example, the cell polarity machinery of Saccharomyces cerevisiae. This cellular module exhibits an intriguing resilience: it...journal article 2023
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Glazenburg, M.M. (author), Laan, L. (author)Cellular life exhibits order and complexity, which typically increase over the course of evolution. Cell polarization is a well-studied example of an ordering process that breaks the internal symmetry of a cell by establishing a preferential axis. Like many cellular processes, polarization is driven by self-organization, meaning that the...review 2023
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de Vries, J.J. (author), Laan, Daphne M. (author), Frey, F.F.F. (author), Koenderink, G.H. (author), de Maat, Moniek P.M. (author)Fibrous networks are essential structural components of biological and engineered materials. Accordingly, many approaches have been developed to quantify their structural properties, which define their material properties. However, a comprehensive overview and comparison of methods is lacking. Therefore, we systematically searched for...journal article 2023
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Zwicker, David (author), Laan, L. (author)Phase separation has emerged as an essential concept for the spatial organization inside biological cells. However, despite the clear relevance to virtually all physiological functions, we understand surprisingly little about what phases form in a system of many interacting components, like in cells. Here we introduce a numerical method based...journal article 2022
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Wortel, Meike T. (author), Agashe, Deepa (author), Bailey, Susan F. (author), Bank, Claudia (author), Bisschop, Karen (author), Blankers, Thomas (author), Laan, L. (author), Meijer, Jeroen (author), Tans, S.J. (author)Evolution has traditionally been a historical and descriptive science, and predicting future evolutionary processes has long been considered impossible. However, evolutionary predictions are increasingly being developed and used in medicine, agriculture, biotechnology and conservation biology. Evolutionary predictions may be used for...review 2022
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Liu, H. (author), de Kruijff, R.M. (author), Laan, A.C. (author), Beekman, F.J. (author), van den Heuvel, E.J. (author), Ramakers, R.M. (author), Eelkema, R. (author), Denkova, A.G. (author)A variety of polymer micelles are designed for the delivery of chemotherapeutic drugs to tumors. Although the promise of these nanocarriers is very high, in the clinic the effectivity is rather limited. Determining the in vivo fate of the micelles can greatly help to improve this treatment. Here, a simple and fast chelator-free method for...journal article 2022
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Linne, C. (author), Visco, Daniele (author), Angioletti-Uberti, Stefano (author), Laan, L. (author), Kraft, Daniela J. (author)Reliably distinguishing between cells based on minute differences in receptor density is crucial for cell-cell or virus-cell recognition, the initiation of signal transduction, and selective targeting in directed drug delivery. Such sharp differentiation between different surfaces based on their receptor density can only be achieved by...journal article 2021
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van Weerdenburg, R.J.A. (author), Pearson, S.G. (author), van Prooijen, Bram (author), Laan, Stendert (author), Elias, Edwin (author), Tonnon, Pieter Koen (author), Wang, Zhengbing (author)Multiple tidal inlet systems like the Wadden Sea have long been considered as separated basins, bordered by so-called tidal divides. Recently, it was however shown that fluxes of water and sediment occur over the borders of these basins, especially during wind events. In this paper, the wind-driven fluxes over these borders and the residual...journal article 2021
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Liu, H. (author), Laan, A.C. (author), Plomp, J. (author), Parnell, S.R. (author), Men, Y. (author), Dalgliesh, Robert M. (author), Eelkema, R. (author), Denkova, A.G. (author)Polymeric micelles, due to their easy preparation and versatile properties, have been widely applied as one of the most popular carriers for chemotherapeutic agents. Such micelles primarily prevent the leakage of drugs during transportation and thus protect healthy tissue. Controlled drug release, which releases the drugs at the site of...journal article 2021
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Sinha, V. (author), Laan, Jochem J. (author), Pidko, E.A. (author)Rapid and accurate prediction of reactivity descriptors of transition metal (TM) complexes is a major challenge for contemporary quantum chemistry. The recently-developed GFN2-xTB method based on the density functional tight-binding theory (DFT-B) is suitable for high-throughput calculation of geometries and thermochemistry for TM complexes...journal article 2021
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Daalman, W.K. (author), Sweep, E. (author), Laan, L. (author)A bottom-up route towards predicting evolution relies on a deep understanding of the complex network that proteins form inside cells. In a rapidly expanding panorama of experimental possibilities, the most difficult question is how to conceptually approach the disentangling of such complex networks. These can exhibit varying degrees of...review 2020
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Laan, Rianne (author), Nout, Remi A. (author), Dankelman, J. (author), van de Berg, N.J. (author)Background:<br/> Brachytherapy involves placement of radioactive sources inside or near the tumour. For gynaecological cancer, recent developments, including 3D imaging and image-guided adaptive brachytherapy, have improved treatment quality and outcomes. However, for large or complex tumours, target coverage and local control with commercially...journal article 2019
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Vendel, K.J.A. (author), Tschirpke, S. (author), Shamsi, F. (author), Dogterom, A.M. (author), Laan, L. (author)Cell polarity - the morphological and functional differentiation of cellular compartments in a directional manner - is required for processes such as orientation of cell division, directed cellular growth and motility. How the interplay of components within the complexity of a cell leads to cell polarity is still heavily debated. In this...review 2019
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Liu, H. (author), Carter, Philippe J.H. (author), Laan, A.C. (author), Eelkema, R. (author), Denkova, A.G. (author)A great number of fluorescent probes have been developed for detecting singlet oxygen (<sup>1</sup>O<sub>2</sub>), which is considered to be one of the most effective reactive oxygen species (ROS), especially in clinical applications. The commercially available fluorescent probe Singlet Oxygen Sensor Green (SOSG) is widely used due to its...journal article 2019
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Jelenic, Ivan (author), Selmecki, Anna (author), Laan, L. (author), Pavin, Nenad (author)Faithful chromosome segregation, driven by the mitotic spindle, is essential for organismal survival. Neopolyploid cells from diverse species exhibit a significant increase in mitotic errors relative to their diploid progenitors, resulting in chromosome nondisjunction. In the model system Saccharomyces cerevisiae, the rate of chromosome loss...journal article 2018