Multiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency

Understanding the impact of guide RNA (gRNA) and genomic locus on CRISPR-Cas9 activity is crucial to design effective gene editing assays. However, it is challenging to profile Cas9 activity in the endogenous cellular environment. Here we leverage our TRIP technology to integrate ~ 1k barcoded reporter genes in the genomes of mouse embryonic...

Large variety in a panel of human colon cancer organoids in response to EZH2 inhibition

EZH2 inhibitors have gained great interest for their use as anti-cancer therapeutics. However, most research has focused on EZH2 mutant cancers and recently adverse effects of EZH2 inactivation have come to light. To determine whether colorectal cancer cells respond to EZH2 inhibition and to explore which factors influence the degree of response...

Deep water winch using synthetic fiber rope

Parallel Natural Language Parsing: From Analysis to Speedup

Het optimaliseren van de cascobouw

Manieren tot verbeteren van de cascobouw

Industrierobots in station 1 bij GNM, een modelsimulatie

Singapore, een centrum van scheepsreparatie

Glasvezelversterkt polyester en de toepassing in

Het Keppel reparatiebedrijf en waarom het daar beter gaat

De Groninger Scheepsbouwproblematiek, in het kader van het College Werfinrichting en Werfbedrijf