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van Oene, M.M. (author), Ha, S. (author), Jager, T. (author), Lee, M. (author), Pedaci, F. (author), Lipfert, J. (author), Dekker, N.H. (author)
Single-molecule manipulation techniques have provided unprecedented insights into the structure, function, interactions, and mechanical properties of biological macromolecules. Recently, the single-molecule toolbox has been expanded by techniques that enable measurements of rotation and torque, such as the optical torque wrench (OTW) and...
journal article 2018
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van Oene, M.M. (author), Dickinson, L.E. (author), Cross, B. (author), Pedaci, F. (author), Lipfert, J. (author), Dekker, N.H. (author)
The bacterial flagellar motor of Escherichia coli is a nanoscale rotary engine essential for bacterial propulsion. Studies on the power output of single motors rely on the measurement of motor torque and rotation under external load. Here, we investigate the use of magnetic tweezers, which in principle allow the application and active control...
journal article 2017
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Van Oene, M.M. (author), Dickinson, L.E. (author), Pedaci, F. (author), Köber, M. (author), Dulin, D. (author), Lipfert, J. (author), Dekker, N.H. (author)
Superparamagnetic beads are widely used in biochemistry and single-molecule biophysics, but the nature of the anisotropy that enables the application of torques remains controversial. To quantitatively investigate the torques experienced by superparamagnetic particles, we use a biological motor to rotate beads in a magnetic field and demonstrate...
journal article 2015
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Lipfert, J. (author), van Oene, M.M. (author), Lee, M. (author), Pedaci, F. (author), Dekker, N.H. (author)
journal article 2015
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Pedaci, F. (author), Huang, Z. (author), Van Oene, M. (author), Dekker, N.H. (author)
The optical torque wrench is a laser trapping technique that expands the capability of standard optical tweezers to torque manipulation and measurement, using the laser linear polarization to orient tailored microscopic birefringent particles. The ability to measure torque of the order of kBT (?4 pN nm) is especially important in the study of...
journal article 2012
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