Precision Engineering of the Co-immobilization of Enzymes for Cascade Biocatalysis

The design and orderly layered co-immobilization of multiple enzymes on resin particles remain challenging. In this study, the SpyTag/SpyCatcher binding pair was fused to the N-terminus of an alcohol dehydrogenase (ADH) and an aldo-keto reductase (AKR), respectively. A non-canonical amino acid (ncAA), p-azido-L-phenylalanine (p-AzF), as the...

Removal of Microcystis aeruginosa by UV-activated persulfate: Performance and characteristics

Cyanobacteria blooms in source waters have become a worldwide issue for drinking water production. UV-activated persulfate (UV/PS) technology was firstly applied to remove cultivated Microcystis aeruginosa (M. aeruginosa) in bench scale. The presence of persulfate significantly enhanced both cytoclasis and algal organic matter mineralization...